姜黄素逆转P—gp介导卵巢癌多药耐药机制的研究 |
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引用本文: | 梁若笳,蒋学禄,姚庆华,姚志韬,张婷,蒋军.姜黄素逆转P—gp介导卵巢癌多药耐药机制的研究[J].实用全科医学,2014(6):932-934. |
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作者姓名: | 梁若笳 蒋学禄 姚庆华 姚志韬 张婷 蒋军 |
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作者单位: | 浙江省中医院妇科,杭州市310006 |
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基金项目: | 2012年浙江省中医药科学研究基金计划(A类)(2012ZA053) |
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摘 要: | 目的探讨姜黄素对P糖蛋白(P—gP)介导的卵巢癌多药耐药的逆转作用及其可能机制。方法采用二苯基溴化四氮唑蓝(MTT)法检测人卵巢癌癌细胞株加药后的增殖,碘化丙啶(PI)染色法检测细胞凋亡率,western blot法检测P—Akt和P-gP的表达量。结果姜黄素合用顺铂处理细胞(OVCAR-3/DDP细胞株)48h,顺铂的浓度在0.05—5μg/ml时,加入姜黄素25μmol/L,耐药细胞生存率下降明显(P〈0.05),尤以顺铂0.25μg/ml和姜黄素25μmol/L作用时耐药细胞生存率下降最明显。单用顺铂组细胞的凋亡率为(13.2±2.5)%,采用在顺铂基础上联合使用姜黄素后细胞凋亡率为(21.8±3.5)%,与单独使用顺铂相比,顺铂与姜黄素联合使用诱导细胞凋亡的作用显著增强(P〈0.05)。与单用顺铂0.25μg/ml相比,联合使用姜黄素后p-Akt和P-gP的表达量明显降低(P〈0.05),2组间Akt的表达量差异无统计学意义(P〉0.05)。结论姜黄素不增加顺铂对OVCAR-3细胞毒性作用;对于OVCAR-3/DDP细胞株,姜黄素明显增加顺铂细胞毒性;姜黄素明显抑制了p-Akt和P—gP蛋白的表达。姜黄素可能通过抑制Akt信号通路降低P—gP的表达进而逆转多药耐药。
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关 键 词: | 姜黄素 P糖蛋白 顺铂 多药耐药 逆转 |
Effect of curcumin on reverse P-gp mediated mechanism of multidrug resistance in ovarian cancer |
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Institution: | LIANG Ruo- fia,JlANG Xne-lu, YAO Qing-hua,et al.( Department of Obstetrics and Gynecology,the First Affiliated Hospital of Zhefiang Traditional Chinese Medicine University ,Hangzhou 310006 ,Zhefiang , China) |
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Abstract: | Objective To investigate the effects of curcumin on P glycoprotein (P-gp) reversal effect of ovarian cancer mediated muhidrug resistance and its possible mechanism. Methods For determination of drugs in vitro cytotoxicity by MT]" assay, apoptosis was detected by PI staining, expression of p-Akt and P-gp for detection by Western blot method. Re- suits Curcumin did not increase cisplatin cytotoxicity on OVCAR-3 ; for OVCAR-3/DDP cells, curcumin significantly in- creased the cytotoxieity of cisplatin;inhibition of expression of p-Akt and P-gp protein were inhibited by curcumin. Con- clusion Curcumin can inhibit Akt signaling pathway by decreasing the expression of P-gp and reversal of multidrug re- sistance. |
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Keywords: | Curcumin Pglycoprotein Cisplatin Multidrug resistance Reversal |
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