胰腺组织的P物质与急性胰腺炎轻重的关系

目的 研究急性胰腺炎时胰腺组织P物质的变化规律。水肿性和出血/坏死性胰腺炎时P物质水平，观察中药复方MCP-1对P物质水平的影响。方法 皮下注射蛙皮缩胆囊肽（caerulein)诱发大鼠急性水肿性胰腺炎，胆总管插管注射牛磺胆酸钠诱发急性出血/坏死性胰腺炎，以免疫组化方法检测P物质。结果 水肿性和出血性/坏死性胰腺炎，2h时SP免疫反应性明显增强，轻型和重型胰腺炎的SP免疫反应性无明显差别；MCP-1使SP免疫反应性明显减弱。结论 P物质在轻型和重型胰腺炎中所起作用相同。MCP-1可能通过降低P物质含量。发挥其抗局部微循环损伤作用。

Relationship Between Level of Pancreatic Substance P and the Severity of Acute Pancreatitis

Objective To investigate the change in pancreatic substance P immunoreactivity during acute pancreatitis, the difference in immunoreactivity between edematous and hemorrhagic/necrotizing pancreatitis, and to observe the effect of Chinese medicine(MCP-1) on SP immunoreactivity. Methods Acute edematous and hemorrhagic/necrotizing pancreatitis were induced in rats by subcutaneous injection of caerulein and intraductal injection of sodium taurocholate respectively. Pancreatic SP level was determined with immunohistological analysis.Results For acute edematous and hemorrhagic/necrotizing pancreatitis, pancreatic SP immunoreactivity was increased significantly at hour 2, and there was no signicant difference between them. Chinese medicine significantly attenuated SP immunoreactivity. Conclusions Substance P plays a similar role in the development of mild and severe types of acute pancreatitis; MCP_1 may exert its effect on pancreatic microcirculation by reducing pancreatic substance P.