VCAM-1在大鼠心脏移植慢性排斥模型中的表达

目的：研究血管细胞粘附分子-1(VCAM-1)在心脏移植慢性排斥反应中的表达及作用。方法：采用供体脾细胞（SPC）和环磷酰胺（CP）预处理移植受体，然后行异位心脏移植术，采用免疫组织化学法检测移植心脏中VCAM-1的表达。结果：SPC和CP预处理后，移植心脏的存活时间明显延长，VCAM-1的表达水平明显减低；而VCAM-1在急性排斥反应和环孢霉素A（CsA）治疗组中的表达均较SPC和CP预处理组明显增强（P＜0.01）。急性排斥反应与CsA治疗组比较，其差异无统计学意义（P＞0.05）。结论：VCAM-1的表达水平与排斥反应的程度成正相关，测定受体VCAM-1的水平可以预测移植器官的功能状况，从而为慢性排斥反应的早期诊断和预防提供依据。

Expression and significance of the vascular cell adhesion molecule-1 in cardiac allografts of rats

Objective: To investigate the expression of the vascular cell adhesion molecule-1(VCAM-1) in allograft chronic rejection(CR) after heart transplantation in rats.Methods: The recipient was pretreated with inoculation of donor splenocytes(SPC) followed by cyclophosphamide(CP).Heterotopic cervical heart transplantation was performed.The cardiac allografts were harvested at different stages after heart transplantation.The immunohistochemical method was used to detect the expression of VCAM-1 in the allografts.Results: Preconditioned with SPC followed by CP,the cardiac allografts was prolonged in their survival.Both faint VCAM-1 expression and less lymphocyte infiltration were observed in the SPC and CP-pretreated group.Controversially,the proteins of VCAM-1 were expressed at a high level and significant lymphocyte infiltration was seen in both the non-treated group and the Cyclosporin A(CsA)-treated group.There was no significant difference in the expression of VCAM-1 between the non-treated group and the CsA-treated group(P>0.05).Conclusion: The expression level of VCAM-1 is associated with the occurrence and the development of rejection and has positive correlation with the severity of graft rejection.The function of allografts may be predicted and it provides evidence for early diagnosis and prevention of CR by detecting VCAM-1.