离体培养时bFGF对顺铂耳蜗毒性的拮抗作用

目的 研究不同浓度的顺铂对耳蜗基底膜毛细胞的损伤、不同浓度的bFGF对顺铂耳蜗基底膜毛细胞损伤的保护作用以及caspase-9的表达。方法 对耳蜗基 底膜进行离体培养，用荧光染色方法对毛细胞数量和caspase-9的表达进行观察。结果 在不同浓度中，15μg/ml顺铂对基底膜毛细胞损伤最大；bFGF浓度≥100ng/ml时，对顺铂所致的基底膜毛细胞损伤具有良好的拮抗作用。结论 顺铂对耳蜗基底膜上毛细胞的损伤具有剂量依赖性； bFGF拮抗顺铂所致的耳蜗基底膜毛细胞的损伤；caspase-9的表达与bFGF对顺铂耳蜗毒性的拮抗作用相关。

BFGF prevents Cisplatin induced hair cell death and Caspase-9 activation in rat cochlea in vitro

Objective Cisplatin of different concentrations can induce hair cell death in the cochlea. To explore if the bFGF of different concentrations prevents ciplatin induced hair cell death and the expression of caspase-9. Method Basilar membranes were cultured and the number of hair cells and the expression of caspase-9 were determined by immunofluorescent staining. Results Cisplatin of 15μg/ml induced the maximal hair cell death, when it was equal to or higher than 100ng/ml, bFGF could well prevent ciplatin induced hair cell death. Conclusions Hair cell death induced by cisplatin is concentration dependent. BFGF can prevent ciplatin induced hair cell death, which is related to the expression of caspase-9.