人体子宫内膜血管内皮生长因子的研究

目的 :对人体子宫内膜增殖期 (n =3)、分泌期 (n =6 )和增生过长 (n =6 )标本 ,进行VEGF的免疫定位和半定量测定 ,了解雌激素对子宫内膜VEGF的影响。方法 :研究组用免疫组织化学方法 ,对照组用无关的免疫球蛋白代替第一及第二抗体 ,用辅助视频图像分析系统进行计算。结果 :增殖期VEGF在腺体 (P <0 .0 0 1)和间质 (P <0 .0 1)的浓度 ,明显高于分泌期和子宫内膜增生过长 ,总的看来 ,腺体的VEGF高于间质。结论 :雌激素提高子宫内膜的VEGF的含量 ,并与增殖晚期新的血管形成和血管通透性增加有关。此外 ,子宫内膜增生过长的腺体VEGF并不升高 ,提示人体子宫内膜的腺体生长和血管生成调节是不同的。

Immunohistological Localisation of Vascular Endothelial Growth Factor in Human Endometrium

Objective:To study immunolocalize and assess semi quantiatively VEGF immunostaining in cells of proliferative phase (n=3),secretory phase (n=6) and hyperplastic (n=6) human endometrial samples in order to understand influence of estrogen on VEGF.Methods:Immunohistochmistry were used to immunolocalize and assess semi quantiatively VEGF.Specificity of antibody liganding and visualization was assessed by omitting primary antibody and secondary antibody replacing primary antibody and secondary antibody with immunoglobulins from same species.Results:VEGF concentrations were significantly higher in glandular (P<0.001) and stromal(P<0.01) compartments of proliferative stage endometrium compared with those in secretory stage and hyperplastic endometrial samples,with no difference in the scores for glandular and stomal compartmentsbetween secretory stage and hyperplastic endometrial samples.Conclusion:Endometrial VEGF expression and concentration are enhanced by estrogen,and may be correlated with neovascularization and increased vascular perneability during late proliferative period.Additionally,there was no enhancement in VEGF expression in hyperplastic gland,suggesting that regulation of glandular growth and that of angiogenesis in human endometrium operate through different mechanisms.