兔颈总动脉损伤后普罗布考对血管再狭窄的抑制作用

目的 研究普罗布考减轻动脉成形术后再狭窄的作用.方法 将30只新西兰大白兔随机分为普罗布考治疗组和对照组各15只.以球囊损伤颈总动脉,用高胆固醇食物饲养8周后切取颈总动脉,HE染色观察血管管腔面积和内膜面积的变化.采用明胶酶谱法和酶法测定动物血清中基质金属蛋白酶2的酶原(pro-MMP-2)、基质金属蛋白酶2(MMP-2)及血清胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)的水平.结果 普罗布考组TC、LDL-C、TG和HDDC水平较对照组低,但差异无显著性(P＞0.05);普罗布考组pro-MP-2、MMP-2水平较对照组低,两组比较均具有显著性差异(P＜0.05);普罗布考组颈总动脉新生内膜面积、最大内膜厚度明显低于对照组,两者比较差异具有显著性(P＜0.05);而普罗布考组颈总动脉管腔面积明显高于对照组后,两者比较差异具有显著性(P＜0.05).结论 普罗布考可通过抑制MMPs的途径减轻兔颈总动脉成形术后再狭窄的形成.

Effects of Probucol on restenosis after rabbit carotid artery balloon injury

Objective To investigate the effect of Probucol on restenosis after carotid artery balloon injury.Methods 30 New Zealand White rabbits were randomly assigned to Probucol group(n=15)and control(n=15).Carotid artery tissues were harvested at weeks 8 after injury by balloon.During 8 weeks the rabbits were fed with hypercholesterolaemic diet.The areas of lumen of blood vessel and intima were observed by HE staining.The levels of serum pro-MMP-2 and MMP-2 were detected by zymograph and total cholesterol(TC),triglyceride(TG),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C) were measured by enzyme method. Results Serum levels of TC,TG,HDL-C,LDL-C,pro-MMP-2 and MMP-2 in probucol group were lower than those in control,respectively;the expression levels of TC,TG,HDL-C and LDL-C in serum had no significant difference between two groups(P>0.05);there were obvious differences of pro-MMP-2,MMP-2 between two groups(P<0.05);there was a significant decrease in intimal area,intimal thickness and a significant increase in luminal area compared control with Probucol group(P<0.05).Conclusion Probucol can prevent restenosis after rabbits carotid artery balloon injury by inhibiting MMPs.