广州及周边地区蝙蝠携带SRAS冠状病毒的流行病学调查

目的了解野生动物市场果蝠携带SARS冠状病毒（SARS-CoV）和SARS样冠状病毒（SARS-like-CoY）情况。方法2004年9月至2005年11月，在广州及广州野生动物市场收集来自广州及周边地区的分属两个亚目9个种的蝙蝠共905只，有犬蝠（Cynopterus sphinx）、棕果蝠（Rousettus leschenaulti）、普通长翼蝠（Miniopterus schreibersi）、普氏蹄蝠（Hipposideros prmti）、中华菊头蝠（Rhinolophus sinicus）、小黄蝠（Scotophilus kuhlii）、大耳双色蹄蝠（Hipposideros Pomona）、中菊头蝠（Rhinolophus affinis）和小菊头蝠（Rhinolophus pusillus）。收集的蝙蝠分别取得咽拭813份、血清524份、肺组织853份及直肠粪便853份标本共计3043份，用新型冠状病毒（N-蛋白）测定试剂盒（酶联免疫法）、SARS-CoV RNA检测试剂盒、荧光PCR及基因芯片检测并通过RT-PCR和细胞分离培养检测SARS-CoV和SARS-like-CoV。结果收集的9个种905只蝙蝠共3043份标本未检测、分离出SARS-CoV和SARS-like-CoV。结论调查的广州及周边地区的9种蝙蝠均未检测到SARS-CoV和SARS-like-CoV。

An epidemiological investigation of bats carrying SARS-CoV in Guangzhou and its vicinity

Objective To detect serve acute respiratory syndrome-associated coronavirus (SARS-CoV) and SARS-like-CoV in fruit bats captured in Guangzhou and its vicinity. Methods Totally 927 bats of 9 species (Cynopterus sphinx, Rousettus leschenaulti, Miniopterus schreibersi, Hipposideros pratti, Rhinolophus sinicus, Scotophilus kuhlii, Hipposideros Pomona, Rhinolophus affinis, and Rhinolophus pusillus) captured in Guangzhou and its vicinity from September 2004 to November 2005 were available for this investigation, from which 3 043 samples (813 throat swasb, 524 sera, 853 lung tissues and 853 colorectal tissue specimens) were obtained. SARS-Cov and SARS-like-CoV were detected in these specimens using diagnostic kit for novel coronavirus N protein (ELISA), SARS-CoV Virus RNA detection kit, fluorescence PCR, Genchip, RT-PCR and cell isolation culture methods. Result and Conclusion No SARS-CoV and SARS-like-CoV were detected in the 3043 samples, indicating the current absence of SARS-CoV and SARS-like-CoV in the bats captured in Guangzhou and its vicinity.