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| 与海洛因依赖有关的特异性mRNA研究 | |
| 引用本文： | 蒙华庆,王广新,刘先俊,周歧新,邓伟.与海洛因依赖有关的特异性mRNA研究[J].重庆医科大学学报,2003,28(5):625-627. |
| 作者姓名： | 蒙华庆 王广新 刘先俊 周歧新 邓伟 |
| 作者单位： | 1. 重庆医科大学临床学院精神科,重庆,400016 2. 山东省济宁医学院附属第二医院,济宁 3. 重庆医科大学基础医学院生化教研室,重庆,400016 4. 重庆医科大学基础医学院药理学教研室,重庆,400016 |
| 基金项目： | 重庆市科委应用基础基金资助项目 ( 99-16-110 ) |
| 摘 要： | 目的：寻找与海洛因依赖有关的特异性基因。方法：用递增法建立海洛因依赖小鼠模型。纳洛酮催瘾后，分别提取实验组及对照组小鼠脑内的总RNA、mRNA，然后进行抑制性消减杂交，杂交产物扩增，凝胶电泳分析。结果：实验组小鼠脑内mRNA含量高于对照组，并于实验组小鼠脑内分离出差异表达的cDNA片段。结论：海洛因依赖小鼠脑内可能存在特异性基因表达。 |
| 关 键 词： | 海洛因依赖 mRNA 抑制性消减杂交 |
| 文章编号： | 0253-3626(2003)05-0625-03 |
| 修稿时间： | 2002年9月17日 |
A study on specific mRNA related to the heroin dependence |
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| MENG Huaqing,et al.A study on specific mRNA related to the heroin dependence[J].Journal of Chongqing Medical University,2003,28(5):625-627. | |
| Authors: | MENG Huaqing |
| Abstract: | Objective:To find out the specific gene related to heroin dependence.Methods:Heroin-dependent models in mice were established by dose-increasing administration.After withdrawal syndrome was precipitated by naloxone,total RNA and mRNA were isolated from the brains of experimental and control groups respectively,then suppressive subtractive hybridization was proceeded.The hybridization sample was amplified by PCR and analyzed on a agarose/EB gel.Results:The mRNA level of the experimental group was significantly higher than that of control group and a differently expressing cDNA fragment was obtained from the brain of the experimental group.Conclusion:There is probably an expression of the specific gene in the brain of heroin-dependent mice. |
| Keywords: | Heroin dependence mRNA Suppressive subtractive hybridization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |