川芎嗪对缺氧缺糖损伤的血管内皮细胞周期和前列环素的影响

目的：研究在缺氧缺糖条件下，血管内皮细胞活性、前列环素生成和细胞周期的变化及川芎嗪对它们的影响。方法：缺氧缺糖诱导血管内皮细胞损伤，MTT比色法、流式细胞仪观察川芎嗪对血管内皮细胞作用及细胞周期的影响，放射免疫法测定细胞培养液中前列环素含量。结果：川芎嗪抑制缺氧缺糖引起的血管内皮细胞减少，其作用主要使S期和G2M期细胞比率增加，促进损伤的血管内皮细胞释放前列环素。结论：川芎嗪具有保护缺氧缺糖诱导血管内皮细胞损伤，其作用主要是通过增加S期和G2M期细胞和前列环素的水平。

Effect of Ligustrazine on Cell Cycle and Prostacyclin of Injured Vascular Endothelial Cells Induced by Hypoxia and Lack of Glucose

Objective: To study the effects of ligustrazine on cell cycle and prostacyclin of injured human umbilical vein vascular endothelial cell line induced by hypoxia and lack of glucose Methods: The experiments were performed by culturing vascular endothelial cell induced by hypoxia and lack of glucose in vitro Cell viability was assessed by MTT assay and cell cycle was observed by flow cytometry Radioimmunoassay (RIA) was used to assess the amount of prostacyclin (PGI 2) Results: The vascular endothelial cell viability and PGI 2 were decreased in condition of hypoxia and lack of glucose injury Preincubation of vascular endothelial cell with Ligustrazine for 24 h significantly increased the cell viability, S Phase cell, G 2M phase cell and PGI 2 level Conclusion: These results demonstrated that ligustrazine had protective effect on hypoxia and lack of glucose injured vascular endothelial cell and the effect may be related to increasing cell viability, S phase cell, G 2M phase cell and level of PGI 2