脑源性神经营养因子在猫备用背根节的表达变化

为探讨脑源性神经营养因子（ＢＤＮＦ）在备用背根节（ＤＲＧ）的表达变化情况，对２０只成年雄性家猫（其中１５只行单侧Ｌ６备用根手术后分别存活３、６、１２天）Ｌ６ＤＲＧ行ＢＤＮＦ免疫组化染色。结果：①正常猫Ｌ６ＤＲＧ神经元以胞体直径３２μｍ和４４μｍ为分界划分出大中小三类；②正常Ｌ６ＤＲＧ内有５０．８７％的神经元呈ＢＤＮＦ阳性反应，备用根手术侧其ＢＤＮＦ阳性神经元数量增多，反应增强，以术后６天变化最显著

Expressional change of brain-derived neurotrophic factor in adult cat spared DRG]

This investigation was aimed to search the expressional change of BDNF in the normal and spared L6DRG. The L6DRGs from 20 adult male cats (5 normal cats, 15 unilateral L6 spared roots cats allowed to survive 3 days, 6 days and 12 days respectively) were embedded in paraffin, using immunohistochemical reaction to detect BDNF. RESULTS: 1. Normal DRG neurons were divided into three categories(large, medium and small) with the cell body diameter 32 microns and 44 microns as the dividing lines. 2. There are 50.87% BDNF positive neurons in normal L6DRG. After operation, the number of BDNF positive neurons of the operated side DRG significantly increased, especially 6 days(76.70%). Normal and postoperative BDNF positive neurons were mainly small neurons. After partial section of DRG, the expression of BDNF in spared DRG neurons increased, which might be involved in promoting collateral sprouting of spared root in spinal cord and rescuing the dorsal horn neurons from partial deafferentation.