一种人FLT3配基同源异型蛋白在组织中的分布

目的研究人ＦＬＴ３配基膜外区缺失１３９ｂｐ的同源异型蛋白在组织中的分布特点。方法采用聚合酶链或逆转录聚合酶链反应法对ＦＬＴ３配基在人肝脏、脑、骨髓、肌肉、肾脏、正常人外周血及４种白血病细胞系中的分布进行了研究，并利用ＤＮＡ测序法鉴定ＰＣＲ产物。结果除脑中未见ＦＬＴ３配基表达外，在肝脏、肌肉、骨髓、肾脏、胎肝、正常人外周血细胞及白血病细胞系中均可检测到ＦＬＴ３配基，而膜外区缺失１３９ｂｐ的ＦＬＴ３配基同源异型蛋白只分布于骨髓、胎肝中。结论ＦＬＴ３配基膜外区缺失１３９ｂｐ的同源异型蛋白分布于造血组织中，可能与造血调控有关。

Localization of a FLT3 ligand isoform in human tissues

Objective To explore the localization of a human FLT3 ligand isoform deleted 139bp of extracellular region. Methods Localization of the FLT3 ligand isoform in human tissues was studied by using PCR and RTPCR. The PCR products were identified by DNA sequencing. Results FLT3 ligand was expressed in liver, fetal liver , bone marrow, muscle, kidney and peripheral blood cells and in four leukemia cell lines, but not in brain, while the FLT3 ligand isoform only in fetal liver and bone marrow. Conclusion The FLT3 ligand isoform was expressed in hematopoietic cells and might be involved in hematopoiesis regulation.