糖毒清对糖尿病肾病大鼠肾组织VCAM-1表达影响的研究

目的:探讨糖毒清治疗糖尿病肾病肾功能不全的可能机制。方法:采用5/6肾切除合中等剂量的STZ腹腔注射方法建立糖尿病肾病肾功能不全模型,将造模成功的大鼠50只随机分为5组:模型组(生理盐水2mL/d灌胃)、糖毒清高剂量组[糖毒清6.75g/(kg·d)灌胃];糖毒清中剂量组[糖毒清4.5g/(kg·d)灌胃]、糖毒清低剂量组[糖毒清2.25g/(kg·d)灌胃]、尿毒清组[尿毒清2.25g/(kg·d)灌胃]。另外10只正常对照组大鼠予生理盐水2mL/d灌胃;治疗10周后,通过免疫组化的方法检测各组大鼠肾组织的VCAM-1表达情况。结果:模型组大鼠肾组织VCAM-1表达明显增多,而糖毒清组大鼠肾组织VCAM-1的表达明显减少。结论:糖毒清可以减少糖尿病肾病肾功能不全大鼠肾组织VCAM-1的过度表达,从而达到控制或延缓糖尿病肾病肾功能恶化的进程。

Experimental study on the Influence of Tang-du-qing Granules on the Expressions of VCAM-1 in Renal Tissues of Model Rats with Diabetic Renal Insufficiency

Objective: To clarify the possible mechanisms of Tangduqing granules through examining and comparing the expressions of VCAM-1 in renal tissues of model rats with diabetic renal insufficiency (DRI). Methods: Sixty male SD rats were experienced combination of 5/6 nephrectomy and abdominal cavity injection with midium-dose streptozotocin to establish model rats with DRI. Fifty successful model rats with DRI were divided into five groups randomly: Model Group (Administered with NS 2mL/d).High Dose Group [Administered with Tangduqing 6.75g/(kg·d)]、Medium Dose Group [Administered with Tangduqing 4.5g/(kg·d)]、Low Dose Group [Administered with Tangduqing 2.25g/(kg·d)]、 Niaoduqing Group [Administered with Niaoduqing 2.25g/(kg·d)]. Ten age-matched healthy male SD rats were served as controls(Administered with NS 2mL/d). Ten weeks later, the expressions of VCAM-1 in renal tissues of rats with DRI were observed through immunohistochemical method.Results: The expressions of VCAM-1 in renal tissues of rats with DRI were obviously enhanced. The expressions of VCAM-1 in renal tissues of rats with Tangduqing were obviously decreased。Conclusion :Tangduqing might reduce the over-expression of VCAM-1 in renal tissues of rats with DRI, and control or delay the development of renal failure.