Interleukin-10 promoter microsatellite polymorphisms influence the immune response to heparin and the risk of heparin-induced thrombocytopenia |
| |
Authors: | Pouplard Claire Cornillet-Lefebvre Pascale Attaoua Redha Leroux Dorothée Lecocq-Lafon Carinne Rollin Jérôme Grigorescu Florin Nguyen Philippe Gruel Yves |
| |
Institution: | a Department of Hematology-Hemostasis, University Hospital of Tours, Franceb GICC UMR 6239 CNRS, University Francois Rabelais, Tours, Francec Laboratory of Hematology, University Hospital of Reims, Franced UPRES-EA 2070. University of Reims, Francee Molecular Endocrinology Laboratory. Institut Universitaire de Recherche Clinique, Montpellier, France |
| |
Abstract: | IntroductionHeparin-induced thrombocytopenia (HIT) results from an atypical immune response with synthesis of IgG antibodies (Abs) to platelet factor 4/heparin complexes (PF4/H), and probably involves both B and T cells. We investigated whether 3 single nucleotide polymorphisms (SNPs), rs1800896 (− 1082G/A), rs1800871 (− 819C/T) and rs1800872 (− 592C/A) and the polymorphic CA repeat microsatellites IL10R 5325CA(11_15)] and IL10G 8134CA(14_29)] are associated with the synthesis of Abs to PF4/heparin and HIT.Materials and methodsEighty-two patients with definite HIT and two control groups were studied. The first control group (Abneg) consisted of 85 patients without Abs to PF4/heparin after cardiopulmonary bypass (CPB). The second control group (Abpos) consisted of 84 patients who had developed significant levels of PF4-specific antibodies after CPB, but without HIT.ResultsAllele frequencies of the 3 SNPs were similar in HIT patients and controls. Fourteen alleles in IL10G (G16 to G29) and 3 alleles in IL10R (R13 to R15) were defined. The short G20 allele of IL10G was more frequent in Abneg patients (8.2%) than in Abpos (2.9%) and HIT patients (3%). It thereby appeared to protect against developing Abs to PF4/heparin (OR 0.29; 95% CI 0.12-0.70], p = 0.006). Combined haplotypes cH1/cH8 comprising the short G20 + R13 alleles were less frequent in HIT (OR 0.33; 95% CI 0.11-0.97], p = 0.036), and levels of Abs to PF4 in Abpos patients were lower in cH1/cH8 subjects (p = 0.019).ConclusionThese results suggest that IL10 promoter microsatellite polymorphisms might influence the immune response against PF4/heparin and the risk of HIT. |
| |
Keywords: | heparin thrombocytopenia IL10 gene polymorphisms |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|