环丙沙星在止血带下家兔肢体骨与肌肉组织中浓度变化的实验研究

目的探讨环丙沙星在止血带下家兔肢体骨与肌肉组织中的浓度变化,为围手术期预防性应用抗生素提供参考。方法经兔耳缘静脉灌注环丙沙星,于灌注给药后30、60、90、120min以止血带阻断家兔肢体血液循环,并于阻断血液循环后30、60、90min检测肢体骨与肌肉组织中环丙沙星浓度。结果家兔肢体骨与肌肉组织中环丙沙星浓度试验组与对照组之间比较,差异有统计学意义（P〈0．05）;在试验组内,相同止血时机、不同止血时间之间比较差异无统计学意义（P〉0．05）;相同止血时间、不同止血时机之间比较差异有统计学意义（P〈0．05）。结论止血带阻断家兔肢体血液循环对环丙沙星在肢体骨与肌肉组织中分布有显著性影响,肢体血液循环阻断后对环丙沙星在骨与肌肉组织中分布无显著性影响,止血时机是影响环丙沙星在骨与肌肉组织中分布的主要因素。

An experimental study on the changes of ciprofloxacin concentration in bone and muscle tissues of the rabbit after tourniquet inflation

Objective To investigate the changes of ciprofloxacin concentration in bone and muscle tissues of the extremity of the rabbit after tourniquet inflation. Methods The blood circulation of the extremity of the rabbit was blocked by tourniquet inflation at different times, separately 30 minutes, 60 minutes, 90 minutes or 120 minutes after administration of eiprofloxacin. Then the ciprofloxaein concentration in bone and muscle tissues was measured separately 30 minutes, 60 minutes, or 90 minutes after tourniquet inflation. Results The changes of the concentration of ciprofloxacin were significantly different between the experimental group and the control group （ P 〈 0. 05 ）. In the experimental groups, those changes were significantly different when the timing of tourniquet inflation was different （P 〈 0. 05 ）. However, the differences were not significant in the situation of the same timing but different duration （ P 〉 0.05 ）. Conclusion Blocking the blood circulation of the extremity of the rabbit with tourniquet can significantly influence the distribution of ciprofloxacin in bone and muscle tissues. However, the influence is not significant once the blood circulation has been blocked. The timing of blocking the blood circulation is the main factor that influences the distribution of ciprofloxacin in bone and muscle tissues.