| 广西麻鸭乙型肝炎感染病毒动物模型的实验研究 |
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| 引用本文： | 陈兆霓,黄仁彬,罗秀,何巧玲,吕鹏,张士军. 广西麻鸭乙型肝炎感染病毒动物模型的实验研究[J]. 中国医学文摘:老年医学, 2012, 0(4): 289-291 |
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| 作者姓名： | 陈兆霓 黄仁彬 罗秀 何巧玲 吕鹏 张士军 |
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| 作者单位： | 广西医科大学药理学教研室,南宁530021 |
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| 基金项目： | 广西自然科学基金资助项目（编号：桂科基0731066） |
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| 摘 要： | 目的 探讨广西麻鸭作为乙型肝炎病毒感染动物模型的可能性.方法 应用广西1 d龄雏麻鸭经腹腔感染鸭乙型肝炎病毒（DHBV）,13 d后采用实时荧光定量PCR法检测麻鸭血清DHBV含量,筛选出DHBV强阳性鸭.结果 共检测148份麻鸭血清标本,其中DHBV阳性标本136份,阳性率为91.9%.结论 广西麻鸭可作为乙型肝炎病毒感染动物模型.实时荧光定量PCR法检测DHBV敏感性较高,重复性好,可用于DHBV检测.
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| 关 键 词： | 广西麻鸭 动物模型 鸭乙型肝炎病毒 实时荧光定量PCR |
Experimental study of guangxi brown spotted duck as hepatitis B virus infection animal model |
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| Affiliation: | CHEN Zhao-ni,HUANG Ren-bin,LUO Xiu et al. Department of Pharmacology, Guangxi Medical University, Nanning 530021, China |
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| Abstract: | Objective To explore the possibility of Guangxi brown spotted duck as hepatitis B virus infection animal model. Methods One-day old Guangxi brown spotted ducks were infected with duck hepatitis B virus （ DH- BV）, and DHBV strong positive ducks were detected by the real-time fluorescence quantitative PCR （FQ-PCR） at 13 day after the infection of DHBV. Results One hundred and forty-eight serum samples in ducks were tested, and 136 samples （91.9%） were positive for DHBV. Conclusion The Guangxi brown spotted duck may be used as hepatitis B virus infection animal model. FQ-PCR method for detection of DHBV is sensitive, repeatable and practicable. |
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| Keywords: | Guangxi brown spotted duck Animal model Duck hepatitis B virus Real-time fluorescence quantitative PCR |
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