柠檬酸钠与三氧化二砷联合应用对胃癌SGC一7901细胞的作用

目的探讨柠檬酸钠（sodiumcitrate，CT）与三氧化二砷（As：O，，AS）联合应用对胃癌SGC-7901细胞凋亡及作用机制。方法用sodiumcitrate（终浓度为5mmol／L，CT5）和As20，（终浓度依次为5t~mol／L，AS5）处理体外传代培养的胃癌SGC-7901细胞株。应用DAPI细胞核染色和流式细胞仪检测细胞凋亡和细胞周期的变化；RT—PCR法观察抗凋亡相关基因bcl一2表达的变化。结果sodiumcitrate与As：03联合应用相对于单用sodiumcitrate或As203可显著提高诱导胃癌细胞凋亡率（P〈0．05）；与空白组相比，用药后G0／G1期细胞比例下降，G2／M期细胞比例上升，细胞周期阻滞于G2／M期，抗凋亡基因bcl．2表达明显下降。结论sodiumcitrate与As：O，联合应用具有协同抗胃癌细胞的作用，其机制可能与细胞周期阻滞、增强诱导胃癌细胞凋亡以及调控凋亡相关基因的表达有关。

Anti-tumor effect of sodium citrate in combination with arsenic trioxide on gastric cancer cell line SGC-7901in vitro

Objective To study the anti-tumor effects of sodium citrate combined with arsenic trioxide on human gastric cancer cell line SGC-7901 in vitro and to explore its possible mechanism. Methods The passaged and cultured gastric cancer cell line SGC-7901 in vitro were treated with sodium citrate （ final concentration was 5mmol/ L, CTS） and（or） A% 03 （final concentration were 5 μmol/L, AS5 ）. Cell proliferation was observed by fluorescent inversive microscopy; Cell apoptosis was explored by DAPI fluorescent stain, then the cell apoptosis rate and phases of cell cycle were determined by flow cytometry. The expression of apoptosis-related genes of bcl-2 was observed by PtT- PCR. Results Sodium citrate combined with A% 03 had a stronger inhibition effect on human gastric cancer cell line SGC-7901 than sodium citrate or As203 （P 〈 0. 05 ）. Compared with blank group, the cell cycle distribution was al- tered and the ration of Go/G1 phase cell decreased and G2/M phase increased （P 〈 0.05）. After intervened by sodi- um citrate combined with A% 03, the expression of bcl-2 decreased （ P 〈 0. 05 ）, Conclusion Sodium citrate combined with A% 03 has the synergistic effects on gastric cancer cell line SGC-7901, and the mechanisms may be related to cell cycle blocking and apoptosis-inducing effects through regulating the expression of the related genes.