急性白血病免疫表型及阳性细胞比例分析

目的探讨急性白血病(AL)免疫表型特征。方法以CD45/侧向角散射(SSC)设门,对89例AL患者进行多参数流式细胞术免疫分型。结果急性髓细胞白血病(AML)中系列分化抗原总表达率及阳性表达患者的阳性细胞比例为CD13(96.7%,68.6%±22.7%)、CD33(95.0%,72.6%±23.5%)、髓过氧化物酶(cMPO)(90.0%,57.5%±26.9%)、CD117(73.3%,33.7%±14.4%);急性B淋巴细胞白血病(B-ALL)中系列分化抗原总表达率及阳性表达患者的阳性细胞比例为cCD79a(96.2%,69.8%±22.1%)、CD19(96.2%,67.4%±23.2%)、CD10(53.8%,66.7%±15.4%);急性T淋巴细胞白血病(T-ALL)中系列分化抗原总表达率及阳性表达患者的阳性细胞比例为cCD3(100.0%,53.6%±18.9%)、CD7(66.7%,92.0%±0.5%)。结论 AL各亚型间系列分化抗原总表达率及阳性表达患者的阳性细胞比例存在差异,可作为流式细胞术免疫分型诊断的依据。

Analysis on the immunophenotype and positive cell percentages of acute leukemia

Objective To investigate the immunophenotype characteristics of acute leukemia（AL） . Methods CD45/ side scatter （SSC） gating strategy and multiparametric flow eytometry were used to determine immunophenotype of 89 patients with AL. Results In acute myeloid leukemia （AML） , the cluster of antigen total percentages and positive cell percentages in patients with positive expression were CD13 （96.7% ,68.6% ± 22.7% ） , CD33 （95.0% , 72.6% ±23.5% ） , myeloperoxidase （cMPO） （90.0% , 57.5% ± 26.9% ） and CD117 （73.3% , 33.7% ±14.4% ）. In acute B lymphoblastic leukemia （B-ALL） , the cluster of antigen total percentages and positive cell percentages in patients with positive expression were cCD79a （96.2% , 69. 8% ± 22. 1%o ） , CD19 （96.2% , 67.4% ±23.2%） and CD10 （53.8%,66. 7% ± 15. 4%）. In acute T lymphoblastic leukemia （T-ALL）, the cluster of antigen total percentages and positive cell percentages in patients with positive expression were cCD3 （100. 0% ,53. 6% ±18.9%） and CD7（66.7%,92.0% ±0.5%）. Conclusions There are differences of the cluster of antigen total percentages and positive cell percentages in patients with positive expression in all subtypes of AL, which can be used for the reference of the immunophenotype diagnosis by flow cytometry.