Sysmex XE2100血液分析仪散点图对检测异型淋巴细胞的价值

目的探讨存在异型淋巴细胞的血液标本在Sysmex XE2100全自动血液分析仪(简称XE2100)白细胞分类(DIFF)通道散点图的变化规律。方法观察40例异型淋巴细胞>10%和异型淋巴细胞<10%且其图像有明显变化的患者标本在XE2100 DIFF通道的散点图,总结其变化规律。结果存在异型淋巴细胞的标本在XE2100的散点图特定区域出现异常散点图。13例以单核细胞型为主的异型淋巴细胞标本在单核细胞散点图的上方出现异常均匀分布的呈椭圆形或近似长方形的散点图;27例以浆细胞型和幼稚型为主的混合型异型淋巴细胞标本中有19例标本在DIFF通道上淋巴细胞和单核细胞所在区域融合成灰白色并且向上延升,另外8例标本提示淋巴细胞、单核细胞和中性粒细胞在各自的分布区域出现,但其周围散点增多并相互之间有交叉,分界不清。结论对仪器提示有异型淋巴细胞存在的患者,结合血液分析仪的DIFF通道散点图形和血涂片镜检,可以降低漏诊和误诊概率。

The significance of scatter diagram from Sysmex XE2100 hematology analyzer on abnormal lymphocyte detection

Objective To investigate the variation principle of DIFF scatter diagram of Sysmex XE2100 hematology analyzer on abnormal lymphocyte.Methods A total of 40 samples with the percentages of abnormal lymphocyte >10% and <10% having DIFF scatter diagram obvious changes were observed,and the variation principle of DIFF scatter diagram was summarized.Results The samples with abnormal lymphocyte had abnormal scatter diagram by Sysmex-XE2100 hematology analyzer.The 13 samples which mainly existed monocytoid atypical lymphocyte showed abnormally homogeneous scatter diagram described as ellipsoidal or oblong above the scatter diagram of monocyte.Among 27 samples which mainly mixed with plasmacytoid atypical lymphocyte and blast-like atypical lymphocyte,there were 19 samples′scatter diagram turning into greyish white and extended upward in the area of lymphocyte and monocyte on the DIFF channel,and the other 8 samples′scatter diagram showed that lymphocyte,monocyte and neutrophile granulocyte in their respective area,but the scatter increased and overlapped around these areas.Conclusions With the prompt indication of abnormal lymphocyte by the analyzer,the DIFF scatter diagram of hematology analyzer with microscopy on blood smear could reduce the chances of misdiagnosis and misidentified cases.