α-硫辛酸对肥胖大鼠血清同型半胱氨酸的影响

目的观察α-硫辛酸(ALA)对高脂饮食诱导(HFD)的肥胖大鼠血清同型半胱氨酸(Hcy)水平的影响。方法选择Wistar大鼠50只,随机选10只普通饲料喂养作为普通饲料组,其余40只高脂饲料喂养10周后评价肥胖大鼠模型,成模肥胖大鼠再随机分为2组:HFD组和HFD+ALA组,后者腹腔注射ALA(30 mg/kg)2周。测定各组大鼠血清Hcy、空腹血糖(FBG)、空腹胰岛素(FIns)、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、游离脂肪酸(FFA)。采用稳态模型胰岛素抵抗指数(HOMA-IR)计算胰岛素抵抗指数(IRI)结果高脂饮食10周后成功诱导肥胖大鼠模型32只。与普通饲料组比较,HFD组Hcy、FIns、IRI、TC、TG、HDL-C、LDL-C等明显升高(P<0.05),而FBG、FFA无明显差异(P>0.05)。与HFD组比较,HFD+ALA组Hcy、FIns、IRI、TC、TG、HDL-C、LDL-C、FFA水平明显下降,而FBG无明显差异。HFD+ALA组FFA水平明显低于HFD组及普通饲料组(P<0.05)。结论高脂饮食诱导的肥胖大鼠血清Hcy水平升高;ALA能够改善高脂饮食诱导的胰岛素抵抗状态,并降低血清Hcy水平。

Influence of alpha-lipoicacid on serum homocysteine in obese rats

Objective To observe the influence of alpha-lipoicacid （ALA） on serum homocysteine （Hcy） level in obese rats fed with high-fat diet （HFD）. Methods A total of 10 rats were selected randomly from 50 Wistar rats, and were fed as a normal forage group, whereas the other 40 rats received a HFD for 10 weeks. Its obese rat model was evaluated, and obese rats were randomly classified into 2 groups ： HFD group and HFD ＋ ALA group who were injected with ALA for 2 weeks intraperitoneally at a close of 30 mg/kg. The Hcy, fasting blood glucose（ FBG）, fasting insulin （ Fins）, triglyceride （ TG ）, total cholesterol （ TC ）, high density lipoprotein cholesterol （ HDL-C ）, low density lipoprotein cholesterol（LDL-C） and non-esterified fatty acid （ FFA ） in serum were determined. The insulin resistance index （IRI） was calculated homeostatic model assessment of insulin resistance （HOMA-IR）. Results The obese rat models of 32 obese rats were obtained after 10 weeks＇ HFD. Compered to the normal forage group, HFD group gained a higher levels of Hcy, Fins, IRI, TC, TG, HDL-C and LDL-C （P 〈 0.05）. FBG and FFA had no obvious difference between the 2 groups （P 〉 0.05）. Compared to the HFD group, HFD ＋ ALA group had a lower levels of Hcy, Fins, IRI, TC, TG, HDL-C, LDL-C and FFA, and FBG had no obvious difference. FFA levels in HFD ＋ ALA group were significantly lower than those in the HFD group and the normal forage group （ P 〈 0.05 ）. Conclusions Obese rats induced by HFD have a high level of Hcy. ALA can improve insulin resistance, and reduce the serum Hey level.