胆红素和血红蛋白对不同尿白蛋白检测方法结果干扰的研究

目的探讨尿中胆红素(Bil)和血红蛋白(Hb)在不同检测方法中对尿白蛋白检测结果的干扰。方法分别以Bil和Hb作为干扰物,对3种不同检测方法(免疫透射比浊法、免疫散射比浊法和免疫胶体金法)进行尿白蛋白检测的干扰试验。结果不同浓度的Bil和Hb对3种尿白蛋白检测方法的结果均会产生影响,且干扰率会随干扰物浓度的增高而增加。Hb对3种检测方法主要呈负干扰;Bil对免疫透射比浊法和免疫散射比浊法检测结果呈正干扰,而对免疫胶体金法检测结果呈负干扰。当尿液中Hb浓度相当于尿隐血"1+"时,Hb对3种检测方法的干扰率绝对值为6.72%～17.43%;当尿液中Bil浓度相当于Bil"1+"时,3种检测方法的干扰率绝对值为7.89%～22.94%。结论 Bil和Hb均会对尿白蛋白的检测结果造成干扰,在检测可能含有Bil和Hb患者尿液的尿白蛋白时,应考虑这些干扰因素对结果的影响。

Investigation on the interference of bilirubin and hemoglobin for different urinary albumin measurements

Objective To investigate the interference of bilirubin （Bil） and hemoglobin （Hb） in urine on different urinary albumin measurements. Methods Bil and Hb, as interferents, performed interference test by 3 different urinary albumin measurements： immuno-nephelometry, immuno-turbidimetry and immuno-colloidal gold method, respectively. Results Bil and Hb with different concentrations could affect the results of urinary albumin by all the 3 measurements, and the interferents rate increased with the increasing concentration of interferents. Hb mainly generated negative interference by the 3 methods, while the interferences of Bil by immuno-nephelometry and immuno-turbidimetry were positive, and by immuno-colloidal gold method was negative. In the 3 methods, when urine occult blood was ＂ 1 ＋ ＂, the absolute value of interference rate of Hb was 6.72% -17.43%. When urine Bil was ＂ 1 ＋ ＂, the absolute value of interference rate was 7.89%-22.94%. Conclusions Bil and Hb could generate interference on urinary albumin measurements. When detecting urinary albumin from the patients with suspected high concentrations of Bil and Hb in urine, the interference factors should be considered.