肺癌患者血浆及外周血单个核细胞Lunx mRNA检测的临床意义

目的检测肺癌患者血浆及外周血单个核细胞肺特异性X基因(Lunx)mRNA,探讨两者对肺癌辅助诊断的临床意义。方法采用荧光定量聚合酶链反应(PCR)检测肺癌患者、肺良性疾病患者、肺外肿瘤患者及健康人血浆及外周血单个核细胞Lunx mRNA。结果肺癌组患者血浆Lunx mRNA阳性率显著高于肺良性疾病组(χ2=113.10,P<0.01)、肺外肿瘤组(χ2=125.34,P<0.01)和健康组(χ2=100.33,P<0.01);Ⅲ～Ⅳ期肺癌患者血浆Lunx mRNA阳性率高于Ⅰ、Ⅱ期肺癌患者(χ2=7.07,P<0.05)。肺癌组患者外周血单个核细胞LunxmRNA阳性率显著高于肺良性疾病组(χ2=32.79,P<0.01)、肺外肿瘤组(χ2=44.44,P<0.01)和健康组(χ2=44.44,P<0.01);Ⅲ～Ⅳ期肺癌患者外周血单个核细胞Lunx mRNA阳性率显著高于Ⅰ、Ⅱ期肺癌患者(χ2=24.52,P<0.01)。血浆Lunx mRNA检测对肺癌辅助诊断的敏感性高于单个核细胞检测的敏感性(χ2=36.46,P<0.01),血浆检测的阴性预测值高于外周血单个核细胞检测的阴性预测值(χ2=16.37,P<0.01)。结论血浆与外周血单个核细胞Lunx mRNA检测均可用于肺癌的辅助诊断,前者敏感性较后者高。

The clinical significance of Lunx mRNA detection in plasma and peripheral mononuclear cells in patients with lung cancer

Objective To investigate the clinical auxiliary diagnosis significance of lung-specific X gene（Lunx） mRNA detection in plasma and peripheral mononuclear cells in patients with lung cancer.Methods Lunx mRNA in plasma and peripheral mononuclear cells was detected by fluorescence quantitation polymerase chain reaction（PCR） in patients with lung cancer,benign lung disease,extrapulmonary tumor and healthy subjects.Results The positive rate of Lunx mRNA from plasma in lung cancer group was significantly higher than those in benign lung disease group（χ2=113.10,P〈0.01）,extrapulmonary tumor group（χ2=125.34,P〈0.01） and healthy subjects（χ2=100.33,P〈0.01）.The positive rate of Lunx mRNA in plasma in patients with Ⅲ-Ⅳ stages of lung cancer was significantly higher than that in patients with Ⅰ-Ⅱ stages of lung cancer（χ2=7.07,P〈0.05）.The positive rate of Lunx mRNA in peripheral mononuclear cells in lung cancer group was significantly higher than those in benign lung disease group（χ2=32.79,P〈0.01）,extrapulmonary tumor group（χ2=44.44,P〈0.01） and healthy subjects（χ2=44.44,P〈0.01）.The positive rate of Lunx mRNA in peripheral mononuclear cells in patients with Ⅲ-Ⅳ stages of lung cancer was significantly higher than that in patients with Ⅰ-Ⅱ stages of lung cancer（χ2=24.52,P〈0.01）.For the auxiliary diagnosis of lung cancer,the sensitivity of Lunx mRNA detection in plasma was higher than that in mononuclear cells（χ2=36.46,P〈0.01）.The negative predictive value of the Lunx mRNA detection in plasma was higher than that in mononuclear cells（χ2=16.37,P〈0.01）.Conclusions The detection of Lunx mRNA in plasma and peripheral mononuclear cells can be used for the auxiliary diagnosis of lung cancer.The sensitivity in plasma is higher than that in mononuclear cells.