标本溶血对电化学发光免疫法结果的影响

目的探讨标本不同程度溶血对电化学发光免疫法(ECLIA)测定结果的影响。方法将血液标本人为干预成不同程度的溶血标本,使用ECLIA检测各组标本中的甲状腺功能、肿瘤标志物、内分泌激素等共28项指标的浓度并做分析。结果标本溶血可使胰岛素(INS)浓度降低,使叶酸(FA)、神经元特异性烯醇化酶(NSE)浓度升高,重度溶血有使铁蛋白测定结果偏高的趋势,但差异无统计学意义,其余指标测定结果不受溶血的影响。结论除某些项目外大部分溶血标本的ECLIA测定结果没有受到影响。测定INS、FA、NSE时应绝对避免使用溶血标本,以确保结果准确。

Influence of sample hemolysis on electrochemiluminescence immunoassay results

Objective To study the influence of different degrees of sample hemolysis on electrochemiluminescence immunoassay（ECLIA） results.Methods The blood samples were manually intervened into different degrees of hemolysis,and were used for detecting and analyzing the concentrations of 28 indices such as thyroid function,tumor markers,endocrine hormones and so on in various groups of samples.Results Sample hemolysis can reduce the concentration of insulin（INS）,and the concentration of folic acid（FA） and neuron-specific enolase（NSE） were increased.Severe hemolysis can make the results to have a higher trend on determination of ferritin,however,it was not statistically significant,and the determination results of other indices was not affected by hemolysis.Conclusions The results of most hemolytic samples except certain indices are not affected.The determinations of INS,FA and NSE should absolutely avoid hemolytic samples to ensure the accuracy of results.