细菌或真菌对UF-1000i尿沉渣分析仪红细胞检测的影响

目的探讨不同浓度不同种类细菌或真菌对UF-1000i尿沉渣分析仪(简称UF-1000i)红细胞(RBC)测定结果的影响,以对泌尿系统疾病诊断、评估提供更可靠的实验室依据。方法健康体检者尿液标本制作成混合尿基质,将不同浓度泌尿系统感染常见细菌和真菌菌液加入制备混合尿基质,用UF-1000i检测各种浓度菌尿RBC。收集各种RBC浓度梯度病理血尿标本,加入白念珠菌菌液,调整真菌终浓度后用UF-1000i检测各种浓度菌尿RBC。每份标本同时进行显微镜镜检。结果泌尿系统感染常见革兰阴性杆菌(大肠埃希菌、铜绿假单胞菌、肺炎克雷伯菌)、革兰阳性球菌(金黄色葡萄球菌)和低浓度的白念珠菌(<106/mL)对UF-1000i RBC测定结果无明显影响。粪肠球菌制备菌尿中,细菌浓度为109/mL时UF-1000i检测结果显示尿RBC假阳性。白念珠菌制备菌尿中,真菌浓度从106/mL开始UF-1000i检测结果显示尿RBC假阳性。真菌浓度增至108/mL时,导致血尿标本RBC定量结果显著假性增高(P=0.006)。结论 UF-1000i具有独立的双检测通道,在很大程度上减少了细菌对尿RBC检测的干扰。但并不能有效避免高浓度真菌对RBC检测结果的影响,易造成尿RBC检测假阳性结果而影响实验室检测质量,需进行显微镜镜检校正以保证检验结果的准确性。

Influence of different bacteria or fungi on the determination of urinary sediments by UF-1000i urinary sediment analyzer

Objective To study the influence of different bacteria or fungi with different levels on the determination of urinary red blood cell（RBC） count by UF-1000i urinary sediment analyzer（UF-1000i）,so as to provide more reliable laboratory evidence in the diagnosis and follow-up of urinary tract diseases.Methods The urine RBC counts were analyzed by UF-1000i from urine mixture specimens and hematuria specimens with Blastomyces albicans,in parallel with manual microscopy respectively.Results The Gram-negative bacilli（Escherichia coli,Klebsiella pneumonia and Pseudomonas aeruginosa） and Gram-positive cocci（Staphylococcus aureus） isolated from urine specimens of urinary tract infection and low-concentration Blastomyces albicans（〈106 mL） were not impact the urine RBC count detected by UF-1000i.High concentration bacteriuria specimens（=109/mL） of Enterococcus faecalis caused the false-positive RBC count.The urine RBC count determined by UF-1000i was caused false-positive by Candida albicans,when the concentration of fungi was more than 106/mL.The concentration of Candida albicans reached to 108/mL,and the hematuria specimen RBC count would falsely increased（P=0.006）.Conclusions UF-1000i has special bacterial examination channel,and it could prevent the interference with urine RBC count obviously.However,it is hard to prevent the interference of high concentration fungi,which would cause false-positive urine RBC count.It is recommended to correct the RBC count by manual microscopy in order to provide accurate laboratory results.