基于健康人群唾液葡萄糖检测体系的构建

目的为实现对健康人群进行日常的无创血糖监测,构建基于唾液的高灵敏度葡萄糖检测体系。方法通过对反应体系最适pH值、离子强度的筛选及酶活性调节剂柠檬醛的添加,对以2,4,6-三溴-3-羟基苯甲酸(TBHBA)为色原的葡萄糖氧化酶法进行改良。结果新构建的葡萄糖检测体系反应30 min趋于完全,在510 nm最大吸收波长下检测吸光度(A)值,得到优化参数组合为pH值5.8、0.3 mol/L NaCl、1 220μg/mL柠檬醛。葡萄糖检测低限0.006 mmol/L。结论优化后的检测体系灵敏度是原体系约7倍,检测低限为健康人空腹时唾液葡萄糖平均浓度的1/10,完全达到检测健康人唾液葡萄糖浓度的灵敏度要求。

Establishment of saliva glucose detection system for healthy subjects

Objective To establish a high sensitive detection system to detect the glucose level in saliva in order to realize the daily noninvasively blood glucose monitoring for healthy subjects. Methods The method of glucose oxidase-peroxidase with 2,4,6-tribromo-3-hydroxybenzoic acid （TBHBA） as chromogen was modified, in which pH value and the consistency of ion were optimized, and citral was added to adjust the activity of the enzyme. Results The reaction of the new detection system could be completed in 30 min, and the maximal absorbing wavelength of detecting absorbance （A） value was 510 nm. The optimization of the new detection system parameters was pH： 5.8, 0.3 mol/L NaC1 and 1 220 μg,/mL citral. The low limit value of glucose level detection was 0. 006 mmol/L. Conclusions The sensitivity of the optimized detection system is about 7 times more than that of the original system. The low limit value is 1/10 of fasting saliva glucose mean level in healthy subjects. It is sensitive enough to detect the glucose level in saliva of healthy subjects.