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小鼠真皮间充质干细胞与成纤维细胞共培养对胶原分泌和TGF-β1表达的影响
引用本文:毛越苹,郭庆,齐庆,谭国珍,曾凡钦.小鼠真皮间充质干细胞与成纤维细胞共培养对胶原分泌和TGF-β1表达的影响[J].中华皮肤科杂志,2000,42(1):484-487.
作者姓名:毛越苹  郭庆  齐庆  谭国珍  曾凡钦
作者单位:中山大学附属第二医院皮肤性病科,广州,510120;
摘    要:Objective To explore the role of mouse dermis-derived mesenchymal stem cells (mdMSC) on skin repair. Methods mdMSC and human dermal fibroblasts were isolated and identified. Human dermal fibroblasts were cultured alone or eoeultured with mdMSC in Transwell chambers with the density ratio of human dermal fibroblasts to mdMSC being 2/5, 1/1, and 2/1. On day 4 and 8 of culture, the expression levels of hydroxyproline and transforming growth factor-beta (TGF-beta) 1 were measured in the supematant of monoculture and coculture by alkaline hydrolysis and ELISA respectively. Results The level of hydro-xyproline was significantly higher in the supematants of coculture system with a density ratio of 2/5 and 1/1 than that in monoculture supematants of human dermal fibroblasts on day 8 (both P < 0.05). Elevated level of TGF-betal was observed in all coculture supematants on day 8 (all P < 0.01) and in the supernatants of coculture system with a density ratio of 1/1 on day 4 (P < 0.05). There was no significant correlation between the expression level of TGF-betal and hydroxyproline in the coculture supernatants (r = 0.108, P > 0.05). Conclusion In vitro coculture with mdMSC can increase the production of hydroxyproline and TGF-betal by fibroblasts, which may be a mechanism underlying the facilitation of skin repair by mdMSC.

关 键 词:真皮    间质干细胞移植    成纤维细胞    

Effect of eoeulture with mouse dermis-derived mesenehymal stem cells on the secretion of collagen and expression of transforming growth faetor-betal by hnman dermal fibroblasts
MAO Yue-ping,GUO Qing,QI Qing,TAN Guo-zhen,ZENG Fan-qin.Effect of eoeulture with mouse dermis-derived mesenehymal stem cells on the secretion of collagen and expression of transforming growth faetor-betal by hnman dermal fibroblasts[J].Chinese Journal of Dermatology,2000,42(1):484-487.
Authors:MAO Yue-ping  GUO Qing  QI Qing  TAN Guo-zhen  ZENG Fan-qin
Abstract:
Keywords:DermisMesenchymal stem cell transplantationFibroblasts
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