Ultrastructural demonstration of T cells in cutaneous tissue sections using specific anti-human T cell antiserum |
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| Authors: | D. M. MACDONALD D. SCHMITT D. GERMAIN J. THIVOLET |
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| Affiliation: | Laboratoire de Recherche Dermatologique et Immunologique, F. R. A. INSERM 11, Clinique Dermatologique, Hôpital Edouard Herriot, 69374 Lyon Cedex 2, France |
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| Abstract: | The presence of T lymphocytes in the situ in cutaneous infiltrates of contact dermatitis and lichen planus has been demonstrated by an immunoelectronmicroscope technique. A specific anti-human T cell antiserum labelled with peroxidase-conjugated anti-immunoglobulin serum was used to reveal the specific human T lymphocyte antigen (HTLA) which was visualized ultrastructurally. This technique shows that labelling is limited to the surface membrane and allows study of the in situ interrelationship of T cells. |
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