光学相干断层扫描与视网膜电图检测大鼠视网膜光化学损伤

目的 比较频域光学相干断层成像（SD—OCT）检测与闪光视网膜电图（f-ERG）检测在蓝光诱导大鼠视网膜光损伤中的敏感性及其相互关系。方法 建立蓝光诱导大鼠视网膜光损伤模型，分别于照射后1d、3d、7d和14d，以SD—OCT测量视网膜外核层厚度，以f-ERG测量各波的反应振幅，分析大鼠外核层厚度与f-ERG各波反应振幅的关系。结果 蓝光照射后1d、3d、7d和14d大鼠平均视网膜外核层厚度依次为（36．54±3．82）μm、（27．07±2．70）μm、（25．37±2．32）μm和（19．54±1．79）μm，与对照组比较，在3d、7d和14d两组差异有统计学意义（F=8．992，8．838，9．183，P〈0．05）；ERG-a波振幅依次为（268．25±41．12）μv，（178．21±32．70）μv，（153．28±48．23）μv，（144．28±33．57）μv；ERG—b波振幅依次为（608．41±61．61）μv，（489．29±85．94）μv，（463．36±58．47）μv，（390．05±48．94）μv。与对照组相比，各波振幅明显降低，差异均有统计学意义（ERG-a波：F=a4．239，b9．443，b10．187，b10．872，aP〈0．05，bP〈0．01；ERG-b波：F=a3．976，b10．391，b10．787，b11．320，aP〈0．05，bP〈0．01；）。大鼠视网膜外核层厚度与f-ERG各波振幅均具有明显的正相关性（ERG-a：r=0．201，0．247，0．338，0．672，P〈0．05；ERG-b：r=0．131，0．278，0．345，0．578，P〈0．05）。结论 蓝光照射大鼠与对照组相比，f-ERG各波的反应振幅明显较对照组降低，可较早地反映光照导致视网膜功能受损；视网膜外核层厚度明显变薄，与前者具有明显相关性，两者分别从形态学及功能学共同揭示蓝光照射在活体大鼠视网膜中的改变。

Optical coherence tomography and electroretinography in detection of retinal photochemical damage in rats

Objective To analyze and compare the sensitivity and relationship of spectral domain optical coherence tomography （ SD-OCT ） and flash electrophysiological tests in the detection of blue light induced retinal photochemical damage in rats. Methods The rat models of blue light induced retinal photochemical damage were established. They were investigated using SD-OCT to obtain cross-sectional images of the retina at 1 d, 3 d, 7 d and 14 d after blue light irradiation. The outer nuclear layer （ONL） thickness was measured. Flash electroretinography （f-ERG） were performed to evaluate the physiological changes. The relationship between retinal morphological and its functional changes was analyzed. Results At 1 d, 3 d, 7 d and 14 d after light exposure, the mean ONL thickness of rats measured by SD-OCT were （36.54±3.82）μm, （27.07±2.70）μm, （25.37±2.32）μm and （19.54±1.79） μm respectively. The thicknesses of 3 d, 7 d, 14 d were significantly thinner than that of control group （F = 8. 992, 8. 838, 9.183,P〈0.05）. The amplitude of ERG-a wave were （268.25 ±41.12） μv, （178.21±32.70）μv, （153.28 ±48.23）μv and （144.28±33.57）μv at the four time point respectively. The amplitudes of ERG-b wave were （608.41± 61.61 ）μv, （489.29± 85.94）μv, （463.36 ± 58.47） μv and （390.05 ±48.94） μv respectively. Compared with control group, the amplitude of ERG a wave and b wave decreased, and the difference were statistically significant （ ERG-a： F =a4. 239, b9. 443, b10. 187, b10. 872, aP 〈 0.05, bP 〈 0. 01 ; ERG-b： F = a3. 976, b10. 391, b10. 787, b11. 320, aP 〈 0. 05, bP〈 0. 01 ）. A significant linear positive association was identified between the amplitude of ERG a, b-wave and the ONL thickness （ ERG-a： r = 0. 201, 0. 247, 0. 338, 0. 672, P 〈 0.05 ; ERG-b ： r = 0. 131, 0. 278,0. 345,0. 578, P 〈 0.05 ）. Conclusion Compared with control group, the amplitude of f-ERG wave decreased significantly in light exposure group, which enabled