VEGF-C在非小细胞肺癌血清及淋巴液中的表达及意义

目的通过对非小细胞肺癌(NSCLC)患者血清中、淋巴液中血管内皮生长因子C(VEGF-C)水平表达的测定来探讨VEGF-C和VEGFR-3在NSCLC肿瘤淋巴转移中的意义。方法采取肺部良性病变手术患者术前血清及术中采取胸导管内淋巴液作为对照组,肺癌患者术前血清及术中行肺癌根治术前,采胸导管内淋巴液为肺癌组,应用酶联免疫(ELISA)法检测VEGF-C浓度。结果55例NSCLC患者淋巴液中VEGF-C水平较血清中VEGF-C水平显著升高,而二者较肺部良性病变对照组淋巴液血清中VEGF-C浓度显著升高(P均<0.01)。肿瘤淋巴结转移组中淋巴液血清中VEGF-C浓度显著高于未有淋巴结转移患者(P均<0.01)。淋巴结转移组中,淋巴液VEGF-C浓度较血清VEGF-C浓度明显增高(P<0.01)。结论NSCLC患者淋巴液及血清中VEGF-C水平高低与肺癌淋巴结转移有关,对其检测可以作为肺癌侵袭转移的量化指标,特别是对淋巴液VEGF-C的研究,可为抑制肿瘤淋巴管生成的靶向治疗提供理论依据。

Expression and significance of VEGF - C in serum and lymph fluid in non-small cell lung carcinoma

Objective It is to determine the expression of VEGFC in serum and lymph fluid in patients with non-small lung carcinoma(NSCLC) to explore the significance of VEGF-C and VEGFR-3 in the lymph metastasis of NSCLC.Methods Serum before operation and lymph fluid in thoracic duct during operation in patients with benign lesion in lung were gotten as control group,and the serum before operation and lymph fluid in before lung cancer radical correction in patients with NSCLC were gotten as lung cancer group.The content of VEGF-C was determined by ELISA.Results The level of VEGF-C in lymph fluid in 55 cases of patients with NSCLC was obviously higher than that in serum.Both the levels were significantly higher than those in serum and lymph fluid in control group(P<0.01).The content of VEGF-C in lymph fluid and serum in patients with lymph metastasis was obviously higher than that in patients without metastasis in lung cancer group(P<0.01).The content of VEGF-C in lymph fluid in patients with lymph metastasis was significantly higher than that in serum (P<0.01).Conclusion The level of VEGF-C in serum and lymph fluid in patients with NSCLC is correlated with lymph metastasis.So the level can be used as quantizing index of metastasis of lung cancer,especially the study on VEGF-C in lymph fluid can provide theory evidence for target treatment for tumor production in lymphatic vessel.