Effectiveness of KTP laser versus 980 nm diode laser to kill Enterococcus faecalis in biofilms developed in experimentally infected root canals |
| |
Authors: | Umberto Romeo DDS Gaspare Palaia PhD Alessia Nardo DDS Gianluca Tenore PhD Vito Telesca DDS Roly Kornblit DDS Alessandro Del Vecchio PhD Alessandra Frioni BScD Piera Valenti BScD Francesca Berlutti BScD |
| |
Affiliation: | 1. Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, Rome, Italy;2. Department of Public Health and Infectious Diseases, Section of Microbiology, Sapienza University of Rome, Rome, Italy;3. CNIS: Research Center for Nanotechnology Applied to Engineering, Sapienza University of Rome, Rome, Italy |
| |
Abstract: | This study aimed to evaluate the antibacterial action of KTP (potassium‐titanyl‐phosphate) laser irradiations (compared with 980 nm diode laser), associated with conventional endodontic procedures, on Enterococcus faecalis biofilms. Fifty‐six dental roots with single canals were prepared with Ni‐Ti rotary instruments, autoclaved, inoculated with an E. faecalis suspension and incubated for 72 h. They were randomly allocated to control and treatment groups. Laser parameters were as follows: power 2.5 W, Ton 35 ms, Toff 50 ms (KTP laser); power 2.5 W, Ton 30 ms, Toff 30 ms (980 nm diode laser). To evaluate the residual bacterial load, BioTimer Assay was employed. The chemo‐mechanical treatment together with laser irradiations (KTP and 980 nm diode lasers) achieved a considerable reduction of bacterial load (higher than 96% and 93%, respectively). Regarding both laser systems, comparisons with conventional endodontic procedures (mortality rate of about 67%) revealed statistically highly significant differences (P ≤ 0.01). This study confirms that laser systems can provide an additional aid in endodontic disinfection. |
| |
Keywords: | BioTimer Assay endodontic infections Enterococcus faecalis biofilm laser root canal disinfection |
|
|