倒卵叶五加总皂苷对心肌缺血再灌注大鼠左心功能和血清一氧化氮及血浆内皮素的影响

目的　观察倒卵叶五加总皂苷对心肌缺血再灌注大鼠左心功能和血清一氧化氮 (NO)及血浆内皮素 (ET)的影响。方法　建立在体大鼠缺血再灌注损伤模型 ,用心功能分析系统测定左室心功能 ,用酶法测定血清NO水平及放免法测定血浆ET水平。实验分假手术组 (sham)、心肌缺血再灌注损伤组 (IR)和倒卵叶五加总皂苷 (SAOH ,10 0mg·Kg-1)治疗组。结果　IR组左室LVSP ,+dp/dtmax,-dp/dtmax,+dp/dtmax/IP明显下降 (P <0 .0 0 1) ,血清NO水平明显降低 ,而血浆ET水平则显著升高(P <0 .0 0 1) ;SAOH能够明显改善缺血再灌注损伤大鼠左心LVSP ,+dp/dtmax,-dp/dtmax,+dp/dtmax/IP ,提高血清NO ,降低血浆ET ,恢复NO/ET平衡 (P <0 .0 0 1)。结论　倒卵叶五加总皂苷能提高心肌缺血再灌注损伤大鼠血清NO水平和降低血浆ET含量 ,恢复NO/ET平衡 ,改善左心舒缩功能。

Effects of saponin of Acanthopanax obovatus on the left cardiac function and the serum level of nitric oxide and the plasma level of endothelin during myocardial ischemic reperfusion injury in rats

OBJECTIVE To study the effects of saponin of Acanthopanax Obovatus Hoo(SAOH) on the left cardiac function,the serum level of nitric oxide(NO) and the plasma level of endothlin(ET) during myocardial ischemic reperfusion injury(IRI) in rats. METHOD The experiment was carried out with IRI of rat in vivo. The left cardiac function was measured by the analysis system,the level of NO in serum by spectrophoto metry and the level of ET in plasma by radioimmunoassay(RIA). The rats were divided into three groups:sham,ischemic/reperfusion(I/R) and SAOH group. RESULTS Compared with the sham group, the left cardiac function, the level of NO and ratio of NO/ET in serum in the I/R group were decreased, while the content of ET in plasma was increased after IRI ( P <0.001). Compared with the I/R group,in the SAOH group, the left cardiac function was improved , the level of NO in serum was increased obviously while the content of ET in plasma was decreased markedly and the ratio of NO/ET was improved ( P <0.001).CONCLUSION SAOH can improved left cardiac function and enhanced the level of NO in serum, decreased the content of ET in plasma after myocardial IRI. One of the mechanism may be that SAOH can enhance NOs secretes and protect myocardial and endothelia cells, rehabilitate the balance between NO and ET.