艾灸对急性胃黏膜损伤大鼠HSP60、HSP70表达的影响

目的观察艾灸中脘、足三里穴对急性胃黏膜损伤模型大鼠胃黏膜上皮HSP60、HSP70表达的影响。方法 32只SD大鼠完全随机分为空白组、模型组、艾灸穴位组和艾灸非穴位组。艾灸预处理大鼠8 d无水乙醇灌胃,造急性胃黏膜损伤模型。采用免疫组织化学法检测HSP60、HSP70的表达。结果与空白组比较,模型组中胃黏膜损伤指数、HSP60表达明显升高(P<0.01)。与模型组和艾灸非穴位组相比,艾灸穴位组胃黏膜损伤指数明显降低(P<0.01),HSP60、HSP70过度高表达(P<0.01)。结论艾灸中脘、足三里穴预处理能够降低无水乙醇对胃黏膜的损伤指数,诱导HSP60、HSP70的过度表达,减少伤害性刺激,从而起到保护胃黏膜的作用。艾灸穴位要比非穴位的作用更加明显,说明艾灸对胃黏膜保护作用具有一定的穴位特异性。

The effluence on impression of heat shock protein 60,heat shock protein 70 in acute gastric mucosal lesion after pro-moxibustion

【Objective】 To observe the effects of moxibustion at Zhongwan(RN12)-Zusanli(ST36) on apoptosis of gastric mucosal cells and the expressions of heat shock protein 60(HSP60) and heat shock protein 70(HSP70).【Methods】 32 SD rats were randomly allocated to blank,model,moxibustion acupoint,moxibustion non-acupoint groups with 8 cases in each group.The rats were pre-treated with moxibustion for 8 days.Acute gastric mucosa injury model was established by anhydrous alcohol-induced method.Test apoptotic cells and expression of HSP60 and HSP70 by radio-immunity.【Results】 Compared with blank group,the expression of gastric mucosal UI and HSP70 in model group increased significantly(P <0.01).While gastric mucosal UI decreased remarkably,HSP60 and HSP70 were over expressed in moxibustion acupoint group compared with model and moxibustion non-acupoint group(P <0.01,P <0.01).【Conclusion】 Pretreatment of moxibustion at Zhongwan(RN12)-Zusanli(ST36) can reduce the injury of gastric mucosal caused by anhydrous alcohol,induce the over-expression of HSP 60 and HSP70,avoid noxious stimulus so as to protect the gastric mucosa which moxibustion acupoint was more potent compared with non-acupoint according to the result.