肌纤维母细胞在先天性肌性斜颈中分布的研究

目的 研究肌纤维母细胞在先天性肌性斜颈（CMT）中的分布及临床意义。方法 利用免疫组化辅以计算机图象定量分析技术。对21例先天性肌性斜颈及10例正常肌肉腱性组织标本α-平滑肌肌动蛋白的表达进行观察和检测。以研究肌纤维母细胞在先天性肌性斜颈（CMT）中的分布。结果 α-SMA在对照组中未见明显表达。3例先天性肌性斜颈标本α-SMA染色阴性，18例病变标本中α-SMA染色阳性，病变组与对照组两者差异有非常显著性意义（P〈0．001）；肌纤维母细胞在肌性斜颈中分布强度与病程有关，6岁以上组分布明显减弱，与6岁以下组（包括6岁）比较差异有显著性意义（P〈0．001）。结论 肌纤维母细胞是先天性肌性斜颈发病关键病理因素。6岁以上组先天性肌性斜颈中肌纤维母细胞的分布明显减弱，而组织纤维化程度加重，似腱样组织。

Distribution of Myofibroblast in Congenital Muscular Torticollis

Objective To study the expression and distribution of myofibroblast in congenital muscular torticollis(CMT) and discuss its clinical significance and relationship with CMT.Methods Twenty-one specimens from CMT were collected between October in 1994 and October in 2002.The control specimens were collected from normal tendon samples.Expression of alpha smooth muscle actin((-SMA) in these specimens was detected by immunohistochemistry and computer image evaluation. Results There was no significant expression of(-SMA in control group except for the positive reaction in the smooth muscle cells of the small vascular walls.Eighteen specimens of CMT were positive for(-SMA.Three specimens of CMT were negative for(-SMA.There was significantly statistical difference of expression of(-SMA between case and control groups(P<0.001).In the case group the expression of(-SMA was associated with the course of CMT.Of those aged over 6,the density of(-SMA was obviously lower than those aged less than 6(including 6) and the difference had statistical significance(P<0.001).Conclusions Myofibroblast is an important factor in the pathogenesis of CMT.The distribution density of myofibroblast in those aged over 6 is going down obviously.And the aligned fibroblasts are surrounded by thick bands of mature collagen fibres,with a tendon-like appearance.