吡拉西坦抗短暂性局灶性脑缺血作用观察

目的 观察吡拉西坦的抗局灶性脑缺血作用。方法采用动脉腔内插线大鼠局灶性脑缺血模型及激光多普勒血流计测定半暗带脑血流、湿重-干重法测定缺血半球水含量、3％伊文氏蓝染色结合图像分析测定血脑屏障(BBB)的破坏、HE染色结合图像分析测定缺血后21h的梗死体积。结果100mg／kg体重吡拉西坦对半暗带脑血流无明显影响，200mg／kg体重吡拉西坦可明显升高再灌注期间半暗带脑血流；100mg／kg和200mg/kg体重的吡拉西坦均可明显降低局灶性脑缺血后缺血半球的水含量及BBB的损伤，缩小梗死体积。结论吡拉西坦具有明确的抗局灶性脑缺血作用，它可改善半暗带脑血流、减轻脑组织水肿和BBB的损伤、缩小梗死体积。

Observation of piracetam against transient local cerebral ischemia

Objective In the study,the protection of piracetam against transient local cerebral ischemia was observed.Methods The rat model of local cerebral ischemia by intraluminal filament was used.The cerebral blood flow (CBF) in penumbra was measured by laser Doppler flowmetry,the water content in ischemic hemisphere was determined by wet-dry weight method and the disruption of blood-brain barrier (BBB) was assessed by Evan's blue staining combined with image analysis.The infarct volume was measured by HE staining combined with image analysis.Results The dose of 200mg/kg,not the dose of 100mg/kg of piracetam,improved the CBF in penumbra.Both doses of piracetam reduced the water content in ischemic hemisphere,decreased the BBB disruption and the infarct volume significantly.Conclusion Piracetam has certain protection against transient local cerebral ischemia.