宫颈脱落细胞中人乳头瘤病毒52型感染及整合状态分析

目的 探讨人乳头瘤病毒(Human papillomavirus,HPV)感染及其整合状况在宫颈病变中的意义.方法 应用HPV L1 通用引物MY09/11 和HPV52 E6 型特异性引物进行多聚酶链反应(Polymerase chain reaction,PCR)检测宫颈脱落细胞标本中HPV 总感染及HPV52 感...

Analysis of human papillomavirus 52 infection and integration status in exfoliated cervical cells

To investigate the importance of human papillomavirus(HPV)infection and integration,HPV and HPV52 positive cervical lesions were detected in cervical exfoliated cells through MY09/11 polymerase chain reaction(PCR)by using HPV L1 primers and HPV52 E6 specific primers.The number of copies of HPV52 E6,E2 and the human housekeeping geneβ-actin was detected with fluorescence quantitative PCR,and the HPV52 integration status was analyzed according to the ratio of E2 to E6.A total of 219 samples from 928 cases of cervical exfoliated cells were HPV positive,and 26 samples from 219 HPV positive cases were HPV52 positive.Among 26 HPV52 positive samples,there were 5 pure episomal cases with E2/E6 ratios=1;14 mixed integrated and episomal cases with an E2/E6 ratio of 0 to 1;and 7 pure integrated cases with an E2/E6 ratio=0.HPV52 integration into the host genome may therefore be a key factor in cervical lesions.The analysis of HPV52 infection and integration status has a certain significance for early screening of patients with cervical lesions.