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支气管肺泡灌洗液中结核分枝杆菌检测的方法比较
引用本文:祖燕 闫国蕊. 支气管肺泡灌洗液中结核分枝杆菌检测的方法比较[J]. 河南医学研究, 1998, 7(3): 259-260
作者姓名:祖燕 闫国蕊
作者单位:河南省结核病防治研究所
摘    要:目的:用刷片、集菌、培养、聚合酶链反应等四种方法检测支气管肺泡灌洗液(BALF)中结核分枝杆菌,对其结果进行比较。方法:采用聚合酶链反应(PCR)法对46例肺结核患者及32例非结核病患者的标本进行检测,并与刷片、集菌、培养法比较。结果:PCR的敏感性显著高于刷片、集菌、培养法。结论:支气管肺泡灌洗液PCR结果阳性,可作为早期诊断结核病的重要指标。

关 键 词:结核病;分枝杆菌;聚合酶链反应

The Comparison of the Methods Detecting Mycobacteriumtuberculosis in Bronchoalvolar Lavage Fluid
Abstract:Objective: The results of dectecting mycobacterium tuberculosis in bronchoalvolar lavage fluid were compared using smear,concentration of bacille,culture and PCR methods.Methods: The samples from 46 patients with tuberculosis,32 patients without tuberculosis were detected using smear,concentrtion of bacille,culture and PCR methods,and the results were compared,Results: The sensitivity of PCR technique was obviously higher than that of others.Conclusion: It may be the important mark in diagnosis early tuberculosis that mycobacterium tuberculosis is detected positivity in BALF.
Keywords:tuberculosis  mycobacterium tuberculosis  polymerase chain reaction
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