慢性萎缩性胃炎不同中医证型模型大鼠胃黏膜细胞增殖基因表达

目的探讨慢性萎缩性胃炎（CAG）不同中医证型与胃黏膜细胞增殖相关基因调控蛋白之间的相关性。方法采用免疫组织化学方法测定CAG不同中医证型模型大鼠中药干预前后胃黏膜细胞增殖相关基因调控蛋白增殖细胞核抗原（PCNA）、表皮生长因子受体（EGFR）及c-myc的表达。结果PCNA、EGFR及c-myc在脾虚型、肝郁型及湿热型cAG大鼠胃黏膜中的表达存在一定的差异，中药干预后cAG不同中医证型模型大鼠胃黏膜PCNA、EGFR及c-myc表达较中药干预前均显著减少。结论CAG胃黏膜细胞增殖调控基因蛋白的表达与中医证型间存在一定的相关性，从而为揭示cAG证型的本质和判定cAG不同中医证型预后提供一定的实验依据。

Experimental Study on Relationship between TCM Syndrome Type and Gastric Mucosa Cell Proliferation in Rat Model of Chronic Atrophic Gastritis

Objective To explore the relationship between TCM syndrome type and gastric mucosa cell proliferation related controlling gene protein in chronic atrophic gastritis (CAG). Methods Expressions of cell proliferation related controlling gene protein, including proliferative cell nuclear antigen (PCNA), epidermal growth factor receptor (EGFR) and c-myc, from gastric mucosa of CAG model rats with different syndrome types were measured by immunohistochemistry and the changes of them before and after TCM intervention were also analyzed by image analysis. Results Protein expressions of PCNA, EGFR and c-myc in gastric mucosa of CAG model rats with different syndrome types (Pi-deficiency type, Gan-stagnation type and dampness-heat type) were different to some extent, and all of them reduced significantly after TCM intervention in the model rats of all syndrome types. Conclusion Expressions of cell proliferation related controlling gene protein in gastric mucosa of CAG model rats of different syndrome types were different to some extent, which provides a certain experimental evidence for revealing the essence of TCM syndrome type of CAG and judging the prognosis of various types.