Neuropeptides in dissociated cell cultures of mammalian spinal cord and dorsal root ganglion |
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Authors: | E. Matthew |
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Affiliation: | The Montreal Neurological Institute and the Department of Neurology and Neurosurgery, McGill University, Montreal, Canada |
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Abstract: | Immunohistochemical studies of leucine-enkephalin, somatostatin, vasoactive intestinal polypeptide and neurotensin were carried out in dissociated cell co-cultures of embryonic mouse spinal cord and dorsal root ganglion, using the peroxidase-antiperoxidase technique. Leucine-enkephalin immunoreactivity exceeded that of the other peptides in these coculture preparations. Leucine-enkephalin, substance P and somatostatin were also studied in spinal cord cultures (without dorsal root ganglia) and in dorsal root ganglia cultures (without spinal cord). Each of these peptides was present in only a small percentage (<10%) of perikarya and processes in spinal cord cultures. No leucine-enkephalin immunoreactivity was seen in dorsal root ganglion cultures; a considerable proportion of the processes were immunoreactive for substance P or somatostatin. These observations suggest that co-cultures of spinal cord and dorsal root ganglia can provide a simplified in vitro “model” of the nervous system for the study of peptidergic interactions. |
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Keywords: | Dissociated cell cultures spinal cord dorsal root ganglia mouse substance P leucine-enkephalin somatostatin neurotensin vasoactive intestinal polypeptide |
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