哮喘患者血浆IL-13、LTB-4、MMP-9、TIMP-1变化及临床意义

目的：探讨哮喘患者血浆IL-13、白三烯B4（LTB-4）、基质金属蛋白酶9（MMP-9）和基质金属蛋白酶组织抑制剂1（TIMP-1）变化及临床意义。方法：选择哮喘患者及查体健康者（对照组）各30例,检测哮喘组急性发作期及稳定期的血浆IL-13、LTB-4、TIMP-1及TIMP-1/MMP-9比值,且与对照组比较;并对哮喘组各检测指标进行相关性分析。结果：哮喘组急性发作期血浆IL-13、LTB-4、MMP-9、TIMP-1及MMP-9/TIMP-1比值明显高于稳定期（P均〈0.05）,上述指标均明显高于对照组（P均〈0.01）;IL-13与LTB-4、MMP-9与TIMP-1、MMP-9、TIMP-1与TIMP-1/MMP-9比值均呈显著正相关（r分别为0.841、0.832、0.921、0.579,P均〈0.05）。结论：IL-13、LTB-4升高及MMP-9/TIMP-1失衡参与哮喘发病,检测IL-13、LTB-4、MMP-9、TIMP-1、TIMP-1/MMP-9比值可评估哮喘病情,指导治疗。

Study on the Significance of Changes of Plasma IL-13,LTB-4,MMP-9,TIMP-1 Levels in Patients with Asthma

Objective To study the significance of changes of plasma IL-13,LTB-4,MMP-9,TIMP-1 levels in patients with asthma.Methods Plasma levels of IL-13,leukotriene B4（LTB4）,MMP-9 and TIMP-1 were determined with ELISA in 30 patients with asthma both on admission and after stabilized as well as once in 30 controls.Results Plasma levels of IL-13,LTB-4,MMP-9 and TIMP-1 in patients with asthma on admission were significantly higher than those in patients stabilized（P〈0.05）and the levels in patients stabilized were still significantly higher than those in controls（P〈0.01）.Conclusion Changes of plasma levels of IL-13,LTB-4,MMP-9 and TIMP-1 could reflect the severity of disease in patients with asthma and monitoring of the changes would be helpful for assessment of the progress of disease.