| 尿毒清颗粒对单侧输尿管梗阻肾间质纤维化大鼠肾脏Toll样受体4表达的影响 |
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| 引用本文： | 胡志娟,贾晓梅,史亚男,郭岚,董春霞,刘冰,牛凯.尿毒清颗粒对单侧输尿管梗阻肾间质纤维化大鼠肾脏Toll样受体4表达的影响[J].中国全科医学,2011,14(30). |
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| 作者姓名： | 胡志娟 贾晓梅 史亚男 郭岚 董春霞 刘冰 牛凯 |
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| 作者单位： | 河北省人民医院肾内科,河北省石家庄市,050051 |
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| 摘 要： | 目的观察尿毒清颗粒对单侧输尿管梗阻模型大鼠肾组织Toll样受体4(TLR4)表达的影响,探讨TLR4在导致肾间质纤维化中的作用。方法将24只大鼠随机分为正常对照组、假手术组、手术组和尿毒清组,每组6只。14 d后处死大鼠,检测各组大鼠的肾功能及总超氧化物歧化酶(T-SOD)活力、丙二醛(MDA)水平。采用反转录聚合酶链反应(RT-PCR)检测转化生长因子-β1(TGF-β1)mRNA的表达水平。采用免疫组化半定量法分析TLR4表达,并观察肾脏病理变化。结果 4组大鼠尿素氮(BUN)、血肌酐(SCr)、T-SOD、MDA水平比较,差异均有统计学意义(P<0.01);4组大鼠TGF-β1mRNA和TLR4的表达水平比较,差异均有统计学意义(P<0.01)。其中,假手术组与手术组、手术组与尿毒清组的TGF-β1mRNA和TLR4表达水平比较,差异均有统计学意义(P<0.05)。结论单侧输尿管梗阻可以诱导大鼠肾间质纤维化,氧化应激可能通过TLR4途径参与肾间质纤维化的形成,尿毒清治疗可明显改善大鼠的肾间质纤维化程度。
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| 关 键 词： | 输尿管梗阻 纤维化 氧化应激 转化生长因子β1 Toll样受体4 |
Impact of Niaoduqing Particles on the Expression of Toll-Like Receptor 4 in Interstitial Fibrosis Kidneys of UUO Rats |
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| HU Zhi-juan,JIA Xiao-mei,SHI Ya-nan,et al..Impact of Niaoduqing Particles on the Expression of Toll-Like Receptor 4 in Interstitial Fibrosis Kidneys of UUO Rats[J].Chinese General Practice,2011,14(30). |
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| Authors: | HU Zhi-juan JIA Xiao-mei SHI Ya-nan |
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| Institution: | HU Zhi-juan,JIA Xiao-mei,SHI Ya-nan,et al.Department of Nephropathy,Hebei General Hospital,Shijiazhuang 050051,China |
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| Abstract: | Objective To explore the changes in toll-like receptor 4(TLR4) expression in kidneys of unilateral ureteral obstruction(UUO) rats and to investigate the function of toll-like receptor 4 in renal interstitial fibrosis.Methods Twenty-four healthy male SD rats were randomly divided into four groups(n=6):normal control group,sham group,UUO group,and Niaoduqing treatment group.Rats were sacrificed at day 14 after surgery,with the activity of T-SOD and levels of MDA measured using chemical colorimetry method.Leve... |
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| Keywords: | Ureteral obstruction Fibrosis Oxidation stress Transforming growth factor beta 1 Toll-like receptor 4 |
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