Rapid identification and sorting of viable virus-reactive CD4(+) and CD8(+) T cells based on antigen-triggered CD137 expression |
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Authors: | Wehler Thomas C Karg Michael Distler Eva Konur Abdo Nonn Marion Meyer Ralf G Huber Christoph Hartwig Udo F Herr Wolfgang |
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Affiliation: | Department of Medicine III — Hematology and Oncology, Johannes Gutenberg-University of Mainz, Langenbeckstr. 1, 55101 Mainz, Germany |
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Abstract: | Current methods for the detection and isolation of antigen-specific CD4+ and CD8+ T cells require the availability of peptide/MHC multimers or are restricted to cells that produce cytokines after antigen contact. Here we show that de novo cell surface expression of the TNF-receptor family member CD137 (4-1BB) identifies recently activated, but not resting, human CD4+ and CD8+ memory T cells. Maximum CD137 expression level is uniformly observed in both T-cell subsets at 24h after stimulation with antigen. In experiments with CMV and EBV-reactive T cells, we confirmed the specificity of CD137 expression by co-staining with peptide/HLA tetramers. Substantial proportions of CD137+ T cells did not produce IFN-γ, suggesting that CD137 detects a broader repertoire of antigen-specific T cells. Activated CD137+ T cells could be easily purified by MACS and expanded in vitro thereafter. This CD137-based enrichment method was capable of isolating 2-fold higher numbers of anti-viral CD4+ and CD8+ T cells compared to the IFN-γ secretion assay. In conclusion, antigen-triggered CD137 expression allows the rapid detection and sorting of virus-reactive CD4+ and CD8+ T cells. The CD137 assay is most attractive for the simultaneous targeting of anti-viral T helper and effector cells in monitoring studies and adoptive immunotherapy trials. |
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Keywords: | CMV, cytomegalovirus EBV, Epstein-Barr virus HD, healthy donor ICS, intracellular cytokine staining IFN, interferon mAb, monoclonal antibody MACS, magnetic-activated cell sorting PBMC, peripheral blood mononuclear cells pHLA, peptide/HLA TNF, tumor necrosis factor |
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