| Grb2的抑制对K562细胞侵袭和迁移能力的影响 |
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| 引用本文： | 姚娜,刘枋,王凌燕,陈慧菁,陈淑萍,叶韵斌. Grb2的抑制对K562细胞侵袭和迁移能力的影响[J]. 福建医科大学学报, 2011, 45(6): 404-408 |
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| 作者姓名： | 姚娜 刘枋 王凌燕 陈慧菁 陈淑萍 叶韵斌 |
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| 作者单位： | 福建医科大学,教学医院、福建省肿瘤医院,肿瘤免疫学研究室、福建省肿瘤转化医学重点实验室,福州,350014 |
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| 基金项目： | 福建省医学创新课题资助项目(2007-CXB-1);国家留学回国人员基金(编号2007-170) |
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| 摘 要： | 目的 探讨Grb2的抑制对K562细胞侵袭和迁移能力的影响.方法 pSilence-4.1CMV-Grb2原核表达载体的构建并应用脂质体介导法将其转染至人慢性髓细胞性白血病细胞K562,后以潮霉素筛选稳定表达siRNA的细胞克隆.应用Western blot和RT-PCR技术,检测转染Grb2 siRNA重组质粒的K5...
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| 关 键 词： | K562细胞 连接蛋白类 受体,生长因子 RNA干扰 细胞运动 肿瘤侵润 |
Effect of Inhibition of Grb2 Expression Level on K562 Cell Invasion and Migration |
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| YAO Na,LIU Fang,WANG Lingyan,CHEN Huijing,CHEN Shuping,YE Yunbin. Effect of Inhibition of Grb2 Expression Level on K562 Cell Invasion and Migration[J]. Journal of Fujian Medical University, 2011, 45(6): 404-408 |
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| Authors: | YAO Na LIU Fang WANG Lingyan CHEN Huijing CHEN Shuping YE Yunbin |
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| Affiliation: | Laboratory of Immuno-oncology,Fujian Provincial Cancer Hospital,Fujian Medical University Teaching Hospital, Fujian Provincial Key Laboratory of Translational Cancer Medicine,Fuzhou 350014,China |
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| Abstract: | Objective To study the effect of inhibition of Grb2 expression level on K562 cell invasion and migration by RNA interference.Methods Eukaryotic expression vector pSilence-4.1CMV-Grb2 was constructed and stably transfected into chronic myelognous leukemia cells K562 using lipofectamine,K562/pSilence-4.1CMV-Grb2 cell lines stably expressing Grb2 siRNA were established with hygromycin selection.The downregulation of Grb2 by stable expression of Grb2 siRNA was confirmed by Western blot and RT-PCR.Cell migration and invasion was monitored by transwell assay.Results Three cell lines with Grb2 siRNA stable expression were established,including K562/pSilence-4.1CMV-Grb2/405,K562/pSilence-4.1CMV-Grb2/541 and K562/pSilence-4.1CMV-Grb2/215.Grb2 mRNA and protein expression levels in K562/pSilence-4.1CMV-Grb2/405 cells were down-regulated and a decrease in cell migration and invasion was observed in these cells.Conclusions Downregulation of Grb2 in K562 cells could lead to a decrease in cell migration and invasion.Our results suggest that gene therapy targeting Grb2 by siRNA may inhibit tumor metastasis. |
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| Keywords: | K562 cells connexins receptors,growth factor RNA interference cell movement neoplasm invasiveness |
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