| HPLC法测定小鼠血浆中杠柳毒苷的血药浓度 |
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| 引用本文： | 阚红玉,谢跃生,王跃飞,戚爱棣,潘桂湘,刘虹.HPLC法测定小鼠血浆中杠柳毒苷的血药浓度[J].中草药,2008,39(10):1493-1496. |
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| 作者姓名： | 阚红玉 谢跃生 王跃飞 戚爱棣 潘桂湘 刘虹 |
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| 作者单位： | 天津中医药大学中医药研究院,教育部省部共建方剂学重点实验室,天津,300193 |
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| 基金项目： | 天津市科技发展基金，天津市科技发展基金，天津市科技发展基金，天津市科技发展基金 |
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| 摘 要： | 目的建立小鼠血浆中杠柳毒苷的HPLC测定方法,对其药动学进行研究。方法血浆样品中加入内标地高辛,用甲醇沉淀蛋白-C18固相萃取处理,氮气吹干后流动相复溶进样。色谱条件:色谱柱为Agilent Zorbas SB-C18柱(150 mm×4.6 mm,5μm)和保护柱Agilent Zorbax C18柱(12.5 mm×4.6 mm,5μm);流动相:乙腈-水(25∶75);体积流量:1.0 mL/min;柱温:25℃;检测波长:220 nm;进样量:20μL。结果此法空白血浆中无内源性物质干扰样品测定,血药浓度线性范围为0.02~2.0 mg/L(r=0.999 8),最低定量限为0.02 mg/L,平均提取回收率大于81.8%,批内、批间精密度RSD均小于4.64%,准确度试验的结果在101.3%~111.7%。结论本方法简便,准确,灵敏度高,并用于小鼠尾静脉注射杠柳毒苷的药时曲线研究。
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| 关 键 词： | 杠柳毒苷 血药浓度 药动学 HPLC |
| 收稿时间： | 2007/12/10 0:00:00 |
Determination of periplocin concentration in mouse plasma by HPLC |
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| KAN Hong-yu,XIE Yue-sheng,WANG Yue-fei,QI Ai-di,PAN Guixiang and LIU Hong.Determination of periplocin concentration in mouse plasma by HPLC[J].Chinese Traditional and Herbal Drugs,2008,39(10):1493-1496. |
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| Authors: | KAN Hong-yu XIE Yue-sheng WANG Yue-fei QI Ai-di PAN Guixiang and LIU Hong |
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| Institution: | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education Research Center of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education Research Center of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education Research Center of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education Research Center of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education Research Center of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education Research Center of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
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| Abstract: | |
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| Keywords: | periplocin blood concentration pharmacokinetics HPLC |
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