高渗盐溶液对失血性休克大鼠红细胞几何形状的影响

目的:采用微管吸吮技术,观察高渗盐溶液对失血性休克大鼠红细胞几何形状的影响。方法:Wistar大鼠随机分为0.9%NaCl(NS)、7.5%NaCl(HS)、5%NaCl-3.5%NaAc(HSA)3组。10min内放血使平均动脉压降至5.3kPa,维持90min。休克模型复制完成后,分别按4mL/kg体重静脉注入NS、HS、HSA,5min内输完。取血测定休克前、后及给药后的红细胞几何形状。结果:休克后红细胞表/体比值、球度指数无明显变化,红细胞直径较休克前下降非常显著。治疗后,HS组与NS、HSA组比较,表/体比值降低非常显著;HS组和HSA组球度指数非常显著高于NS组,HSA组球度指数非常显著低于HS组。HS组和HSA组红细胞直径非常显著低于NS组。结论:HS和HSA不利于失血性休克大鼠红细胞几何形状的改善。HSA对红细胞几何形状的不利影响低于HS。

Effect of hypertonic saline solution on the geometry of erythrocyte in rats subjected to hemorrhagic shock

AIM: To study the effect of hypertonic saline solution on the geometry of erythrocyte in hemorrhage-shocked rats,using micropippette aspiration technique. METHODS: Wistar rats were randomized into three groups of 0.9 % NaCl(NS),7.5% NaCl(HS) and 5% NaCl-3.5% NaAc(HSA). The animals were bled to reach a mean arterial pressure of 5.3 kPa in 10 minutes and maintained in shock for 90 minutes. 4 mL/kg NS,HS or HSA was given intravenously and respectively in 5 minutes following hemorrhagic shock. The blood was collected to determine the geometry of erythrocyte at baseline,after shock and treatment. RESULTS: There were no significant difference in surface area/volume ratio and spherical index between baseline and shock.The diameter of erythrocyte were decreased obviously following hemorrhagic shock. The surface area/volume ratio was significantly lower in HS group than that in NS and HSA group after treatment. HS and HSA raised spherical index of erythrocyte remarkbly compared with NS.Otherwise,the spherical index of erythrocyte was reduced significantly in HSA group compared to HS group. Both HS and HSA decreased diameter of erythrocyte notably compared with NS. CONCLUSION: Both HS and HSA have deleterious effects on the geometry of erythrocyte in rats subjected to hemorrhagic shock.Whereas, HSA could decrease the side effect compared with HS.