两种兔可控性急性心肌缺血再灌注模型的比较

目的 完善兔可控性急性心肌缺血再灌注(I/R)动物模型的制备,提高模型成功率和动物成活率.方法 成年健康家兔60只,随机分为假手术组30只(Sham组)、I/R1组15只和I/R2组15只.Sham组仅穿线不接扎,I/R1组选择左冠状动脉前降支,I/R2组选择冠状动脉左缘支.开胸后以5-0 prolene线穿过冠状动脉,硅胶管圈套5-0 prolene线,用血管钳收紧硅胶管阻断冠状动脉血流,制备兔可控性心肌缺血再灌注模型.四肢安装肢体导联,通过心电图sT段的改变作为心肌缺血的评定指标.结果 Sham组30只动物手术前后心电图均无变化;I/R1组结扎后,13只动物ST段无明显变化;2只动物ST段轻度压低,再灌注时无心律失常表现;I/R2组15只动物结扎后ST段均抬高明显,并渐与QRS波融合,QRS波幅随着时间的延长逐渐变小,再灌注时11只出现室性早搏,4只出现室性心动过速.结论 兔可控性心肌缺血再灌注模型应选择冠状动脉左缘支.用血管钳收紧硅胶管圈套5-0 prolene线的方法 可以准确地复制兔心肌缺血再灌注的实验模型,简化实验操作步骤.

Controllable acute myocardial ischemia-reperfusion models in rabbits

Objective To establish a novel and simple method for establishing rabbit model of acute myocardial ischemia-reperfusion(ML/R).Methods Rabbits were randomly assigned to 3 experimental groups:Sham-operated control(threading a needle without ligation),I/R1(anterior descending branch of coronary ligation foUowed by reperfusion),L/R2(left marginal branch of coronary ligation followed by reperfusion).ECG was observed.Results A11 ST segments were elevated in I/R2(n:15).The models were developed successfully.Conclusion A novel model was simple and reliable for MI/R study in the rabbit with left marginal branch of coronary ligation.