原发性肝癌患者外周血自然杀伤细胞受体NKG2D的表达及意义

目的 探讨原发性肝癌患者外周血自然杀伤(NK)细胞受体NKG2D表达的变化,并分析其对NK细胞杀伤活性的影响.方法 用流式细胞仪分析20例原发性肝癌、23例乙型肝炎后肝硬化、20例慢性乙型肝炎及20名健康者的外周血NK细胞数量及其NKG2D的表达情况,并用酶标仪检测各组样本NK细胞的细胞毒活性.结果 原发性肝癌患者的NK细胞杀伤率、NK细胞NKG2D的表达率(中位数)、NKG2D+NK细胞数(中位数)、NK细胞中NKG2D的表达水平(中位数)及NK细胞数(25±7)%、6%、0.7×107/L、15、(1.1±0.6)×108/L]均明显低于正常组(63±7)%、36%、8.3×107/L、116、(27±1.1)×108/L]和乙肝组(41±8)%、16%、2.8×107/L,49、(1.9±1.1)×108/L],差异均有统计学意义(均P＜0.05);与肝硬化组(29±10)%、7%、0.6×107/L、29、(1.5±1.2)×108/L]比较也略低,但只有NK细胞NKG2D的表达水平两组差异有统计学意义(P＜0.05),其余两组间差异均无统计学意义(均P＞0.05).NK细胞的活性与NK细胞数、NK细胞NKG2D的表达率、NKG2D+NK细胞数、NK细胞NKG2D的表达水平均呈正相关关系(r=0.657、0.770、0.927、0.734,均P＜0.01).结论 原发性肝癌患者外周血NK细胞活性及其细胞表面NKG2D的表达明显降低,NKG2D的表达与NK细胞的活性密切相关,提高NK细胞表面NKG2D的表达水平可为原发性肝癌的过继免疫治疗开创新的思路.

Expression of NK cells receptor NKG2D from peripheral blood in patients with primary hepatic carcinoma and its clinical significance

Objective To investigate the expression of NK cells receptor NKG2D from peripheral blood in patients with primary hepatic carcinoma and the relationship between NKG2D expression and cytotoxicity of NK cells.Methods Flow cytometry was used to determine the number of NK cells and the expression of NK cells receptor NKG2D from peripheral blood in patients with primary hepatic carcinoma (20 cases) ,hepatitis B cirrhosis (23 cases ),hepatitis B ( 20 cases ) and healthy control ( 20 cases ).The microplate reader was used to detect cytotoxicity of NK cells in all cases.Results Among killing rate of NK cell for K562 cell,the expression rate of NKG2D in NK cells,the number of NKG2D+ NK cells,NKG2D expression level of NK cells and the number of NK cells,the liver cancer group(25 ±7)% ,6% ,0.7 × 107/L,15,(1.1 ± 0.6) × 108/L]decreased significantly as compared with the healthy group (63 ± 7 )% ,36%,8.3 ×107/L,116,(2.7 ±1.1) × 108/L]and the hepatitis B group (41 ± 8)%,16%,2.8 × 107/L,49,(1.9 ± 1.1 ) × 108/L](P ＜ 0.05 ) ;and there was a slight decrease as compared with the hepatitis B cirrhosis group(29 ± 10)% ,7% ,0.6 × 107/L,29,(1.5 ± 1.2)× 108/L](all P ＞0.05 except NKG2D expression level of NK cells P ＜0.05).The activity of NK cells showed a obvious positive correlation with the number of NK cell and the positive rate of NKG2D in NK cells,the number of NKG2D+ NK cells and NKG2D expression level of NK cells (r=0.657,0.770,0.927,0.734,all P＜0.01).Conclusion The cytotoxicity and the NKG2D expression of NK cells decreased significantly from peripheral blood in patients with primary hepatic carcinoma.The activity of NK cells was closely related to the NKG2D expression level of NK cells.Enhancing the NKG2D expression level of NK cell may provide a new idea for adoptive immunotherapy of primary hepatic carcinoma.