EB病毒DNA与鼻咽癌关系的动态研究

目的：探讨EB病毒DNA（EBV—DNA）在鼻咽癌放疗前后的动态变化及与复发、远处转移的关系。方法：采用PCR加限制性内切酶酶切技术检测EBVDNA。结果：放疗前，74例标本中有71例（95．9％）EBV—DNA片段检出阳性；放疗50Gy／5周时，23例鼻咽原发灶和颈部淋巴结消失，其阳性率为13．0％（3／23），余51例肿块未消者阳性率为62，7％（32／51）；放疗至70Gy／7周时，7例放疗后有残留，有残留者在放疗结束时EBV—DNA片段的检出阳性率为71．4％（5／7），在67例肿块消失者中未检出阳性EBV—DNA片段。12例复发者中，11例EBV-DNA片段检出阳性；8例转移者中EBV—DNA片段检出均为阳性。结论：检测血浆EBV—DNA能很好地反映肿瘤的消长，是诊断鼻咽癌残留、复发及远处转移的敏感指标。

The dynamic study between EBV-DNA with nasopharyngeal carcinoma

Objective:To study the varies of blood plasm Epstein-Barr virus(EBV)DNA before and after radiotherapy in nasopharyngeal carcinoma and the relationship between it with the tumor growth and decline,residual and relapse as well as metastasis.Method:Polymerase chain reaction(PCR)with restriction enzyme technique was used to detect EBV-DNA.Result:Seventy-one out of 74 specimens were EBV-DNA positive,the positive rate was 95.9%(71/74).In the time of radiation dose 50 Gy/5 week,the primary nasopharynx tumors and lymph node of neck in 23 cases disappeared,the positive rate was13.0%(3/23).In the group of tumors remained,the positive rate was 62.7%(32/51).In the time of radiation dose 70 Gy/7 week,7 out of 74 existed residual tumor after radiotherapy,5 out of 7 cases were positive,the positive rate was 71.4%(5/7).In the cases the tumors disappeared,EBV-DNA was negative.Eleven out of 12 cases with relapse after radiotherapy EBV-DNA fragments detected were positive.All of 8 patients with metastasis after radiotherapy,their EBV-DNA fragments detected were positive.Conclusion:The detection of blood plasm EBV-DNA can reflect the tumors growth and decline well.It is an important and sensitive index in diagnosing the residual and relapse as well as the metastasis of nasopharyngeal carcinoma.