DPC4基因在胰腺癌中的改变

目的 研究DPC4（deleted in pancreatic cancer locus4)基因在胰腺癌中的改变。方法 用多聚酶链反应－单链构象多态性分析（PCR－SSCP）银染技术及PCR产物直接测序法检测胰腺癌细胞系P1、P2、P3、P4、P7、11例新鲜冷冻胰腺癌组织中DPC4基因第1、2、3、4、8、11外显子的缺失和突变。结果 5株人胰腺癌细胞系中，3株（P1、P2、P3）存在DPC4基因突变，DPC4基因改变率为3／5。11例新鲜冷冻胰腺癌细胞中，3例有DPC4基因的纯合性缺失，2例有基因突变，DPC4基因改变率为5／11（45．5％）。结论DPC4作为一种抑癌基因，其改变在胰腺癌的形成中可能起重要作用。

Alterations in DPC4 gene in pancreatic cancers

Objective To examine the alterations in the DPC4 gene in pancreatic cancers. Methods Five pancreatic adenocarcinoma cell lines (P1, P2, P3, P4, and P7) and 11 fresh frozen pancreatic cancer tissues were monitored with polymerase chain reaction (PCR), single strand conformation polymorphism (SSCP) analysis to demonstrate the sequence of deletion and mutation in 6 exons (1, 2, 3, 4, 8, and 11) of the DPC4 gene. Results Mutation in the DPC4 gene was found in 3 of 5 cell lines (P1, P2, and P3). Three of eleven fresh frozen tissues showed homozygous deletions and two showed intragenic mutations, with a gene alteration frequency of 45.5% (5/11). Conclusion Alterations in tumor suppressor gene DPC4 may play an important role during the tumorigenesis of pancreatic cancer.