亚低温下温敏型骨髓间充质干细胞移植治疗大鼠颅脑创伤

目的 观察在亚低温条件下,向颅脑创伤（TBI）大鼠颅内移植温度敏感型骨髓间充质干细胞（tsSV40LTag-BMSCs）对受损神经功能恢复的影响.方法 将54只成年雄性SD大鼠随机分为对照组、亚低温组、亚低温移植组,每组18只.使用溴脱氧尿嘧啶核苷（Brdu）标记tsSV40LTag-BMSCs.使用液压冲击脑损伤仪制备大鼠TBI模型,将对照组大鼠进行常规处理,对亚低温组、亚低温移植组进行亚低温治疗;采用立体定向引导,向亚低温移植组大鼠颅内进行多点移植标记后的tsSV40LTag-BMSCs;分别于伤后第1、7、14、21、28天,对实验组大鼠进行神经运动功能评分.结果 ①制备TBI模型后,各实验组大鼠死亡率比较,差异无统计学意义（χ2=0.477,P=0.788）;TBI后第1天进行评分,3组大鼠神经运动功能评分比较,差异无统计学意义（F=0.435,P〉0.05）.②在TBI后第7、14、21、28天,亚低温组、亚低温移植组的神经运动功能评分高于对照组;亚低温移植组的神经运动功能评分高于亚低温组,差异均有统计学意义（P〈0.05）.3）3组大鼠的神经运动功能评分均随损伤后时间的延长而升高,差异有统计学意义（P〈0.05）.结论 在亚低温条件下,移植tsSV40LTag-BMSCs治疗TBI大鼠,能够明显改善大鼠脑创伤所致的神经运动功能障碍.

Mild hypothermia-mediated temperature-sensitive bone marrow mesenchymal stern cells for traumatic brain injury in rats

Objective To observe the effect of the transfected temperature-sensitive simian virus 40 large T gene-bone marrow mesenchymal stem cells （tsSV40LTag-BMSCs） on the recovery of impaired neurological function in rats with traumatic brain injury （TBI） under the conditions of mild hypothermia. Methods Fifty-four male aduh SD rats were randomly divided into 3 groups： control, mild hypothermia and mild hypothermia transplantation groups （n = 18 each group）. A rat TBI model was induced by a fluid percussion brain injury device. The rats in the control group were treated conventionally, and the rats in both mild hypothermia and mild hypothermia transplantation groups were treated with mild hypothermia. Multi-spot transplantation of tsSV4OLTag-BMSCs was performed in the rats of the mild hypothermia transplantation group using stereotactic guidance; neuromotor function in the experimental groups was scored at the day 1,7, 14, 21 and 28 after injury. Results （1）After inducing the TBI model, there was no significant difference in mortality rate between the experimental groups （X2 = 0. 477, P = 0. 788 ） ; The first day after TBI, there were no significant differences in neuromotor function scores among the 3 groups （F = 0. 435, P 〉0.05）. （2）At day 7, 14, 21 and 28 after TBI, the neuromotor function scores in the mild hypothermia group and mild hypothermia transplantation group were higher than those in the control group. The neuromotor function scores in the mild hypothermia transplantation group were higher than those in the mild hypothermia group. There was significant difference （P 〈0. 05）. （3）The neuromotor function scores of the 3 groups increased with time. There were significant differences （P 〈 0.05）. Conclusion Mediated under the mild hypothermia, the transplanted tsSV40LTag-BMSCs may significantly improve TBI caused neuromotor dysfunction in rats.