喉鳞癌血管内皮生长因子的表达及其临床意义

目的　研究喉鳞癌组织中血管内皮生长因子 (vascularendothelialgrowthfactor,VEGF)的表达及其临床意义。方法　用免疫组化ABC法对 33例喉鳞状细胞癌和 15例声带息肉标本进行血管内皮生长因子测定和肿瘤微血管密度 (microvesseldensity ,MVD )记数。结果　喉癌组织中VEGF的表达程度与MVD呈正相关(P <0 .0 1) ;喉癌组织中 ,VEGF阳性表达率高于声带息肉组织中的VEGF阳性表达率 (P <0 .0 1) ;VEGF表达与病人年龄、病变部位和喉鳞癌细胞分化程度无关 (P >0 .0 5 ) ;VEGF表达与喉鳞癌T分期和颈淋巴结转移呈正相关 (P <0 .0 1) ,而与病人预后呈负相关 (P <0 .0 1)。结论　VEGF在喉鳞癌中有高表达 ,VEGF的表达与新生血管密切相关 ,VEGF的表达程度与喉鳞癌T分期和颈淋巴结转移有关 ,可作为判断喉鳞癌病人预后的参考指标。

Expression of VEGF in laryngeal squamous cell carcinomas and its clinical significance

Objective To study the expression of vascular endothelial growth factor (VEGF) in laryngeal squamous cell carcinomas (SCC) and its significance. Methods The immunohistochemical ABC method was applied respectively to detect the expression of VEGF and count the microvessel density (MVD) in 33 cases of laryngeal SCC and 15 cases of polyp of vocal cord. Results Laryngeal SCC was VEGF positive. The MVD was associated with VEGF expressed in cytoplasm of tumor cells. VEGF expression was not associated with age of the patients, tumor site and histological grade of differentiation, but positively correlated with T stage and cervical lymph node metastasis, whereas, negatively correlated with survival rate. Conclusion VEGF related to tumor grade and lymph node metastasis in laryngeal SCC, takes part in the angiogenesis of tumor, served as a prognostic factor for the patients.