慢性肝病患者外周血细胞间质胶原酶(MMP-1)及其抑制剂(TIMP-1)基因表达的研究

采用RT-PCR法及Northem杂交，检测38例慢性肝炎患者及20例正常成人外周血单个核细胞中MMP-1及TIMP-1mRNA的表达。结果发现正常成人外周血单个核细胞中存在MMP-1及TIMP-1的表达，慢肝患者外周血单个核细胞中MMP-1及TIMP-1的表达升高，MMP-1/TIMP-1比值显著升高，并且与肝纤维化严重程度呈正相关。与肝纤维化血清学指标HA、LN-C-Ⅳ、PCⅢ变化一致，提示外周血单个核细胞中MMP-1及TIMP-1mRNA的表达水平与肝纤维化严重程度关系密切。MMP-1/TIMP-1比值可为临床诊断提供一个新的血清学指标。

The expression of MMP- 1 and TIMP- 1 in peripheral mononucleocytes of patients with chronic hepatitis

To study the expression of the metalloproteinase MMP - 1 and its inhibitors TIMP - 1 in peripheral mononucleocytes, 38 patients with chronic hepatitis and 20 normal adults were examined by RT - PCR and Northern blot methods. The results showed that MMP - 1 and TIMP - 1 mRNA were expressed in peripheral monoucleocytes of normal adults, and were highly expressed in peripheral mononucleocytes of patients with chronic hepatitis. The results also indicated that the increase of MMP 1 /TIMP - 1 is positive correlated with the fibrotic staging and necroinflammatory grading in chronic hepatitis and accords with the changes of hepatic firbrosis markers including HA, LN, C- IV and PC III. These date suggest that the level of MMP - 1 and TIMP - 1 are closely associated with hepatic fibrosis development and MMP -1/TIMP- 1 maybe useful in clinic as a novel serum marker.